Tested Applications
| Positive WB detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16574-1-AP targets POLR3B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9856 Product name: Recombinant human POLR3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 794-1133 aa of BC046238 Sequence: MLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE Predict reactive species |
| Full Name | polymerase (RNA) III (DNA directed) polypeptide B |
| Calculated Molecular Weight | 1133 aa, 128 kDa |
| Observed Molecular Weight | 128 kDa |
| GenBank Accession Number | BC046238 |
| Gene Symbol | POLR3B |
| Gene ID (NCBI) | 55703 |
| RRID | AB_2168001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NW08 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR3B antibody 16574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POLR3B Polyclonal antibody (Cat# 16574-1-AP) has 1 citation. It was used in a 2023 study published in Cell Reports (IF 7.7), investigating subcellular localization and fate of RNA polymerase III in mouse cells. The antibody was applied for Western blot and immunofluorescence. The research falls within the field of molecular and cell biology, specifically RNA polymerase III biology and subcellular protein localization.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
The single review for this product reports a negative experience. The user tested the antibody for Western Blot at a 1:500 dilution on HEK293T cells and stated it "didn't work at all," giving it a 1-star rating. No additional details or troubleshooting notes were provided.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Krishnendu (Verified Customer) (02-27-2020) | Didn't work at all
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