Tested Applications
| Positive WB detected in | mouse liver tissue, human heart tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16583-1-AP targets CFHR3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9963 Product name: Recombinant human CFHR3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC058009 Sequence: MLLLINVILTLWVSCANGQVKPCDFPDIKHGGLFHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDYIHCTQNGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGNSTEVACHPGYGLPKAQTTVTCTEKGWSPTPRCIRVRTCSKSDIEIENGFISESSSIYILNKEIQYKCKPGYATADGNSSGSITCLQNGWSAQPICINSSEKCGPPPPISNGDTTSFLLKVYVPQSRVEYQCQPYYELQGSNYVTCSNGEWSEPPRCIHPCIITEENMNKNNIKLKGRSDRKYYAKTGDTIEFMCKLGYNANTSILSFQAVCREGIVEYPRCE Predict reactive species |
| Full Name | complement factor H-related 3 |
| Calculated Molecular Weight | 330 aa, 37 kDa |
| Observed Molecular Weight | 45-56 kDa |
| GenBank Accession Number | BC058009 |
| Gene Symbol | CFHR3 |
| Gene ID (NCBI) | 10878 |
| RRID | AB_2080151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. Deletion of CFHR1 and CFHR3 genes was found to be associated with decreased risk of age-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CFHR3 antibody 16583-1-AP | Download protocol |
| IP protocol for CFHR3 antibody 16583-1-AP | Download protocol |
| WB protocol for CFHR3 antibody 16583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-CFHR3 (Cat# 16583-1-AP) has 10 total citations published in journals including Clinical Cancer Research, International Journal of Nanomedicine, Cancer Cell International, Journal of Ethnopharmacology, Molecular Medicine Reports, Scientific Reports, Experimental Eye Research, PLoS ONE, Bioengineered, and Aging. Research fields span hepatocellular carcinoma, complement system regulation, glomerular nephropathies, age-related macular degeneration, atypical hemolytic uremic syndrome, and dry eye disease.
| Species | Application | Title |
|---|---|---|
Clin Cancer Res Complement regulatory proteins CFHR1 and CFHR3 and patient response to anti-CD20 monoclonal antibody therapy. | ||
Int J Nanomedicine Innovative Dual mRNA-Lipid Nanoparticle Therapy Targeting CRHBP and CFHR3 for Enhanced Treatment of Hepatocellular Carcinoma | ||
Cancer Cell Int Identifying disulfidptosis subtypes in hepatocellular carcinoma through machine learning and preliminary exploration of its connection with immunotherapy | ||
J Ethnopharmacol Qiju Dihuang Pill modulates complement system in age-related dry eye disease: Tripartite validation through clinical-omics-animal integration. | ||
Mol Med Rep Complement factor H‑related 3 overexpression affects hepatocellular carcinoma proliferation and apoptosis. | ||
Sci Rep A haplotype in CFH family genes confers high risk of rare glomerular nephropathies. |
