Tested Applications
| Positive WB detected in | human plasma tissue, HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16608-1-AP targets APOF in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9824 Product name: Recombinant human APOF protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 37-326 aa of BC026257 Sequence: TSYGKQTNVLMHFPLSLESQTPSSDPLSCQFLHPKSLPGFSHMAPLPKFLVSLALRNALEEAGCQADVWALQLQLYRQGGVNATQVLIQHLRGLQKGRSTERNVSVEALASALQLLAREQQSTGRVGRSLPTEDCENEKEQAVHNVVQLLPGVGTFYNLGTALYYATQNCLGKARERGRDGAIDLGYDLLMTMAGMSGGPMGLAISAALKPALRSGVQQLIQYYQDQKDANISQPETTKEGLRAISDVSDLEETTTLASFISEVVSSAPYWGWAIIKSYDLDPGAGSLEI Predict reactive species |
| Full Name | apolipoprotein F |
| Calculated Molecular Weight | 326 aa, 35 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC026257 |
| Gene Symbol | APOF |
| Gene ID (NCBI) | 319 |
| RRID | AB_2058253 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13790 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
APOF protein, full name Apolipoprotein F, also known as Lipid transfer inhibitor protein (LTIP), belongs to the apolipoprotein F family. This protein is primarily expressed by the liver and secreted into the plasma. Its core function is to act as a cholesterol transport regulator, modulating the transport of cholesterol among plasma lipoproteins by inhibiting the activity of cholesteryl ester transfer protein (CETP). APOF is initially synthesized as a precursor protein with a theoretical molecular weight of approximately 35.4 kDa. After processing, the mature peptide has a molecular weight of about 17.4 kDa. However, in plasma, due to modifications such as glycosylation, the apparent molecular weight detected experimentally is typically around 33-35 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for APOF antibody 16608-1-AP | Download protocol |
| WB protocol for APOF antibody 16608-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APOF Polyclonal antibody (Cat# 16608-1-AP) has 2 citations. They appear in Cancer Cell International and Frontiers in Molecular Bioscience. Both studies used IHC in human samples and focused on cancer biomarker identification—one investigating potential biomarkers in cholangiocarcinoma and the other identifying DNASE1L3 as a clinical stage biomarker in liver hepatocellular carcinoma. The research fields are hepatobiliary oncology and proteomics-based biomarker discovery.
| Species | Application | Title |
|---|---|---|
Cancer Cell Int Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma. | ||
Front Mol Biosci Identification of DNASE1L3 as a novel biomarker of clinical stage in liver hepatocellular carcinoma. |
