Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, mouse spleen tissue |
| Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16698-1-AP targets NAA40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10092 Product name: Recombinant human NAT11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC052298 Sequence: MGRKSSKAKEKKQKRLEERAAMDAVCAKVDAANRLGDPLEAFPVFKKYDRNGLNVSIECKRVSGLEPATVDWAFDLTKTNMQTMYEQSEWGWKDREKREEMTDDRAWYLIAWENSSVPVAFSHFRFDVECGDEVLYCYEVQLESKVRRKGLGKFLIQILQLMANSTQMKKVMLTVFKHNHGAYQFFREALQFEIDDSSPSMSGCCGEDCSYEILSRRTKFGDSHHSHAGGHCGGCCH Predict reactive species |
| Full Name | N-acetyltransferase 11 (GCN5-related, putative) |
| Calculated Molecular Weight | 237 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC052298 |
| Gene Symbol | NAA40 |
| Gene ID (NCBI) | 79829 |
| RRID | AB_3085505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UY6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
N-alpha-acetyltransferase 40 (NAA40, also known as NatD, Nat4, or Patt1) is a highly specific epigenetic enzyme catalyzing the transfer of an acetyl moiety at the N-terminal end of histones H4 and H2A (PMID: 26666750). NAA40 is significantly upregulated in primary CRC tissues and promotes CRC cell growth both in vitro and in xenograft tumor models (PMID: 30858358). Western blot analysis detected NAA40 at an apparent molecular mass of 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NAA40 antibody 16698-1-AP | Download protocol |
| IHC protocol for NAA40 antibody 16698-1-AP | Download protocol |
| WB protocol for NAA40 antibody 16698-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NAA40 Polyclonal antibody (Cat# 16698-1-AP) has 4 citations published in Science Advances, Cell Biology and Toxicology, Cell Signaling, and Biochemical and Biophysical Research Communications. The associated research fields include histone cotranslational acetylation, colorectal cancer, oral squamous cell carcinoma, and osteosarcoma, with applications primarily in Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Sci Adv Mechanism of cotranslational modification of histones H2A and H4 by MetAP1 and NatD.
| ||
Cell Biol Toxicol RNF112, whose transcription is regulated by KLF4, inhibits colorectal cancer growth via promoting ubiquitin-dependent degradation of NAA40 | ||
Cell Signal N-α-Acetyltransferase 40 promotes oral squamous cell carcinoma progression by enhancing FEN1 transcription and suppressing CD8(+) T cell antitumor immunity. | ||
Biochem Biophys Res Commun N-terminal histone acetyltransferase NAA40 modulates osteosarcoma progression by controlling AGR2 expression |
