Tested Applications
| Positive WB detected in | HepG2 cells, HuH-7 cells, SMMC-7721 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
This antibody works well in ProteinSimple system.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16727-1-AP targets TRMT6 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10176 Product name: Recombinant human TRMT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-497 aa of BC001262 Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS Predict reactive species |
| Full Name | tRNA methyltransferase 6 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC001262 |
| Gene Symbol | TRMT6 |
| Gene ID (NCBI) | 51605 |
| RRID | AB_2878306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJA5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR. This antibody is specific to 56kd TRMT6 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRMT6 antibody 16727-1-AP | Download protocol |
| IP protocol for TRMT6 antibody 16727-1-AP | Download protocol |
| WB protocol for TRMT6 antibody 16727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRMT6 Polyclonal antibody (Cat# 16727-1-AP) has 15 citations published in journals including Nature Immunology, Nature Communications, Cell Discovery, Journal of Experimental Medicine, Cancer Letters, and Cell Death Discovery. Research fields span tRNA/mRNA m1A modification, T cell expansion, liver and lung tumorigenesis, ciliogenesis, pulmonary hypertension, neuroblastoma, colitis, cholesterol metabolism, and epitranscriptome signatures in cancer. Applications include WB, IHC, IP, IF, and RIP.
| Species | Application | Title |
|---|---|---|
Nat Immunol tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis
| ||
Nat Commun N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism. | ||
Acta Pharm Sin B ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17 | ||
Cancer Lett YTHDF3 Drives Tumor Growth and Metastasis by Recruiting eIF4B to Promote Notch2 Translation in Breast Cancer | ||
Small Unveiling Novel Biomarkers: Ferroptosis and m1A in the Progression of Nanographene-Induced Lung Fibrosis. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The user tested it for Western Blot on HepG2 cell lysate at a 1:20 dilution and reported a very good signal, noting they used a simple western (ProteinSimple) setup. Overall, the feedback highlights strong performance and reliable signal quality in routine WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Estefania (Verified Customer) (05-16-2022) | Very good signal. Tested using simple western (ProteinSimple)
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