HSD17B8 Polyclonal antibody

HSD17B8 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16752-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

17 beta HSD 8, D6S2245E, FABG, FABGL, H2 KE6, HKE6, HSD17B8, Ke 6, KE6, Protein Ke6, RING2, SDR30C1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 16752-1-AP
Hela cells lysates were subjected to SDS PAGE followed by western blot with 16752-1-AP (HSD17B8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human testis using 16752-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 16752-1-AP (HSD17B8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 16752-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 16752-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 16752-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 16752-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 16752-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 16752-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, mouse small intestine tissue, human testis tissue
Positive IHC detected in	human liver cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16752-1-AP targets HSD17B8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10226 Product name: Recombinant human HSD17B8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC008185 Sequence: MASQLQNRLRSALALVTGAGSGIGRAVSVRLAGEGATVAACDLDRAAAQETVRLLGGPGSKEGPPRGNHAAFQADVSEARAARCLLEQVQACFSRPPSVVVSCAGITQDEFLLHMSEDDWDKVIAVNLKGTFLVTQAAAQALVSNGCRGSIINISSIVGKVGNVGQTNYAASKAGVIGLTQTAARELGRHGIRCNSVLPGFIATPMTQKVPQKVVDKITEMIPMGHLGDPEDVADVVAFLASEDSGYITGTSVEVTGGLFM Predict reactive species
Full Name	hydroxysteroid (17-beta) dehydrogenase 8
Calculated Molecular Weight	261 aa, 27 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC008185
Gene Symbol	HSD17B8
Gene ID (NCBI)	7923
RRID	AB_2248540
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92506
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HSD17B8 antibody 16752-1-AP	Download protocol
WB protocol for HSD17B8 antibody 16752-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The HSD17B8 Polyclonal antibody (Cat# 16752-1-AP) has 2 citations. One appears in Nature Communications (2023), studying cell facilitation promoting growth and survival under drug pressure in breast cancer. The other is in Cell Reports (2026), investigating how high glucose impairs cognitive function through lipid droplet accumulation via lactylation of HSD17B10. The research fields span oncology (breast cancer biology) and neuroscience (glucose metabolism and cognition).

Species	Application	Title
human	WB	Nat Commun Cell facilitation promotes growth and survival under drug pressure in breast cancer Authors - Rena Emond View Article
		Cell Rep High glucose impairs cognitive function by inducing lipid droplet accumulation through lactylation of HSD17B10 at K105. Authors - Jingxi Xu View Article