KD/KO Validated

RP9 Polyclonal antibody

RP9 Polyclonal Antibody for WB, ELISA

Cat No. 16832-1-AP

Publications (1)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PAP 1, PAP-1, Pim 1 associated protein, Pim-1-associated protein, Retinitis pigmentosa 9 protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Quantity

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Filter: AllWB

WB analysis of HeLa using 16832-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 16832-1-AP
WB result of RP9 antibody (16832-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RP9 transfected HepG2 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 16832-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 16832-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 16832-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 16832-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 16832-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for RP9 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells, HepG2 cells, MCF-7 cells, mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16832-1-AP targets RP9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10441 Product name: Recombinant human RP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-221 aa of BC025928 Sequence: MSSRPGREDVGAAGARRPREPPEQELQRRREQKRRRHDAQQLQQLKHLESFYEKPPPGLIKEDETKPEDCIPDVPGNEHAREFLAHAPTKGLWMPLGKEVKVMQCWRCKRYGHRTGDKECPFFIKGNQKLEQFRVAHEDPMYDIIRDNKRHEKDVRIQQLKQLLEDSTSDEDRSSSSSSEGKEKHKKKKKKEKHKKRKKEKKKKKKRKHKSSKSNEGSDSE Predict reactive species
Full Name	retinitis pigmentosa 9 (autosomal dominant)
Calculated Molecular Weight	221 aa, 26 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC025928
Gene Symbol	RP9
Gene ID (NCBI)	6100
RRID	AB_2878322
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TA86
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RP9 antibody 16832-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The RP9 antibody (Cat# 16832-1-AP) has 1 total citation. It appears in Molecular Cell (IF 16), in a study investigating metazoan-specific splicing factors in the human Step 1 spliceosome. The research field is RNA splicing and spliceosome biology. The citation validated the antibody for Western blot (WB) in human samples.

Species	Application	Title
human	WB	Mol Cell Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome. Authors - Karl Bertram View Article

Reviews

5.0 out of 5

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1 verified customer reviews

What customers say

The single review for this product is highly positive, with a 5-star rating. The user performed a Western Blot on B cells at a 1:1000 dilution and reported clean signal, noting they used a blotting accelerator. Overall, the feedback highlights the antibody's strong performance and clear results in WB applications.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Annabel (Verified Customer) (12-18-2025)

Good antibody, used with blotting accelerator, clean signal