Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF-P detected in | rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16949-1-AP targets CPLX3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10571 Product name: Recombinant human CPLX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-158 aa of BC018026 Sequence: MAFMVKTMVGGQLKNLTGSLGGGEDKGDGDKSAAEAQGMSREEYEEYQKQLVEEKMERDAQFTQRKAERATLRSHFRDKYRLPKNETDESQIQMAGGDVELPRELAKMIEEDTEEEEEKASVLGQLASLPGLNLGSLKDKAQATLGDLKQSAEKCHVM Predict reactive species |
| Full Name | complexin 3 |
| Calculated Molecular Weight | 158 aa, 18 kDa |
| Observed Molecular Weight | 20-23 kDa |
| GenBank Accession Number | BC018026 |
| Gene Symbol | CPLX3 |
| Gene ID (NCBI) | 594855 |
| RRID | AB_2084042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WVH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPLX3, also named as Complexin-3, is 158 amino acid protein, which belongs to the complexin/synaphin family. CPLX3 localizes in the membrane and binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A. CPLX3 positively regulates a late step in synaptic vesicle exocytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CPLX3 antibody 16949-1-AP | Download protocol |
| IP protocol for CPLX3 antibody 16949-1-AP | Download protocol |
| WB protocol for CPLX3 antibody 16949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CPLX3 Polyclonal antibody (Cat# 16949-1-AP) has 4 citations published in Proc Natl Acad Sci U S A, Free Radic Biol Med, Cell Rep, and Neuroscience. The associated research fields span neuroscience and neurobiology, covering topics such as murine subplate cell populations, NOX2 deficiency in cerebral injury, localized mRNA and protein synthesis in cortical layer 1, and electroacupuncture effects on glutamatergic presynaptic deficits.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke. | ||
Neuroscience Electroacupuncture restores maternal separation-induced glutamatergic presynaptic deficits of the medial prefrontal cortex in adulthood |
