Tested Applications
| Positive WB detected in | HEK-293 cells, mouse heart tissue, HeLa cells, HepG2 cells, mouse liver tissue, rat heart tissue, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16969-1-AP targets MRPL20 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9160 Product name: Recombinant human MRPL20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-149 aa of BC009515 Sequence: MVFLTAQLWLRNRVTDRYFRIQEVLKHARHFRGRKNRCYRLAVRTVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPALIGNLVKCQVELNRKVLADLAIYEPKTFKSLAALASRRRHEGFAAALGDGKEPEGIFSRVVQYH Predict reactive species |
| Full Name | mitochondrial ribosomal protein L20 |
| Calculated Molecular Weight | 149 aa, 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC009515 |
| Gene Symbol | MRPL20 |
| Gene ID (NCBI) | 55052 |
| RRID | AB_3741901 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MRPL20 (mitochondrial ribosomal protein L20) is a 149-amino-acid nuclear-encoded protein that belongs to the bacterial ribosomal protein bL20 family. As a structural constituent of the mitochondrial ribosome , MRPL20 localizes to the 39S large subunit, where it integrates into the mitochondrial inner membrane and facilitates the synthesis of mitochondrial genome-encoded proteins-including essential transmembrane subunits of the mitochondrial respiratory chain-by participating in all three phases of mitochondrial translation: initiation, elongation, and termination.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPL20 antibody 16969-1-AP | Download protocol |
| WB protocol for MRPL20 antibody 16969-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MRPL20 Polyclonal antibody (Cat# 16969-1-AP) has 2 citations. These appear in Nature Structural & Molecular Biology (2024), covering mitochondrial ribosome assembly in humans, and Bone (2025), addressing extracellular pH regulation of osteoclast fusion and activation in mice. Both studies utilized the antibody for Western blot analysis. The research fields span mitochondrial translation/ribosome biology and bone/osteoclast biology.
| Species | Application | Title |
|---|---|---|
