Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, K-562 cells, Jurkat cells, NIH/3T3 tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16973-1-AP targets FANCC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9423 Product name: Recombinant human FANCC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 206-558 aa of BC015748 Sequence: CHGREPQEILQPEFFEAVNEAILLKKISLPMSAVVCLWLRHLPSLEKAMLHLFEKLISSERNCLRRIECFIKDSSLPQAACHPAIFRVVDEMFRCALLETDGALEIIATIQVFTQCFVEALEKASKQLRFALKTYFPYTSPSLAMVLLQDPQDIPRGHWLQTLKHISELLREAVEDQTHGSCGGPFESWFLFIHFGGWAEMVAEQLLMSAAEPPTALLWLLAFYYGPRDGRQQRAQTMVQVKAVLGHLLAMSRSSSLSAQDLQTVAGQGTDTDLRAPAQQLIRHLLLNFLLWAPGGHTIAWDVITLMAHTAEITHEIIGFLDQTLYRWNRLGIESPRSEKLARELLKELRTQV Predict reactive species |
| Full Name | Fanconi anemia, complementation group C |
| Calculated Molecular Weight | 558 aa, 63 kDa |
| Observed Molecular Weight | 47-50 kDa |
| GenBank Accession Number | BC015748 |
| Gene Symbol | FANCC |
| Gene ID (NCBI) | 2176 |
| RRID | AB_2231524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FANCC antibody 16973-1-AP | Download protocol |
| WB protocol for FANCC antibody 16973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody Anti-53BP1 (Cat# 16973-1-AP) has 1 total citation. It appears in the journal Cell Reports (Impact Factor 7.7), published in August 2025. The associated research field involves DNA damage response, specifically the role of 53BP1 in p53-E2F7-dependent transcriptional gene repression and its participation in the Fanconi anemia pathway. The application used was Western Blot (WB) in human samples.
| Species | Application | Title |
|---|---|---|
Cell Rep 53BP1 regulates p53-E2F7-dependent transcriptional gene repression and participates in the Fanconi anemia pathway.
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