Tested Applications
| Positive WB detected in | human skeletal muscle tissue |
| Positive IHC detected in | mouse spleen tissue, mouse thymus tissue, human tonsillitis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17015-1-AP targets FAM65B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9991 Product name: Recombinant human FAM65B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 205-591 aa of BC001232 Sequence: SIKMKGLAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGKQSWDGEETVFLPLIVGFISIKVTELKGLATHILVGSVTCETKELFAARPQVVAVDINDLGTIKLNLEITWYPFDVEDMTASSGAGNKAAALQRRMSMYSQGTPETPTFKDHSFFSNLPDDIFENGKAAEEKMPLSLSFSDLPNGDCALTSHSTGSPSNSTNPEITITPAEFNLSSLASQNEGMDDTSSASSRNSLGEGQEPKSHLKEEDPEEPRKPASAPSEACRRQSSGAGAEHLFLENDVAEALLQESEEASELKPVELDTSEGNITKQLVKRLTSAEVPMATDRLLSEGSVGGESEGCRSFLDGSLEDAFNGLLLALEPHKEQYKEFQDLNQEVMNLDDILKK Predict reactive species |
| Full Name | family with sequence similarity 65, member B |
| Calculated Molecular Weight | 118 kDa |
| Observed Molecular Weight | 115 kDa, 66 kDa |
| GenBank Accession Number | BC001232 |
| Gene Symbol | FAM65B |
| Gene ID (NCBI) | 9750 |
| RRID | AB_10640810 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4F9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAM65B is also named as C6orf32, DIFF48, KIAA0386, PL48 and belongs to the FAM65 family.It has 2 isoforms produced by alternative splicing.Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation(PMID:17150207). FAM65B is expressed in mononuclear cells, not in myotubes, upon induction of differentiation and cellular fusion, and expression is upregulated during human myoblast differentiation at both the mRNA and protein levels(PMID:17150207).This antibody is specific to the isoform of 119 kDa of FAM65B.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM65B antibody 17015-1-AP | Download protocol |
| WB protocol for FAM65B antibody 17015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-FAM65B antibody (Cat# 17015-1-AP) has 5 total citations published in PNAS, Genome Biology, iScience, FASEB Journal, and Journal of Cell Science. Associated research fields include hair cell stereocilia and hearing, glucocorticoid-stimulated TLR/Rho GTPase signaling in neutrophils, melanoma multinucleation via the RAS/ERK pathway, HDAC6-dysferlin complex formation during myogenic differentiation, and FAM65B-mediated neutrophil polarization.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing. | ||
Genome Biol Quantitative proteomics and phosphoproteomics reveal glucocorticoid stimulation of TLR and Rho GTPase signaling in neutrophil-like cells. | ||
iScience RIPOR2 promotes multinucleation of melanoma cells downstream of the RAS/ERK oncogenic pathway. | ||
FASEB J Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation. | ||
J Cell Sci Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils.
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