Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17034-1-AP targets Methylmalonyl Coenzyme A mutase/MUT in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10523 Product name: Recombinant human MUT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 401-750 aa of BC016282 Sequence: SARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDTVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGVSTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV Predict reactive species |
| Full Name | methylmalonyl Coenzyme A mutase |
| Calculated Molecular Weight | 750 aa, 83 kDa |
| Observed Molecular Weight | 78 kDa |
| GenBank Accession Number | BC016282 |
| Gene Symbol | MUT |
| Gene ID (NCBI) | 4594 |
| RRID | AB_2147263 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22033 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids (PMID: 15647267). Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA (PMID: 30428564), resulting in life-threatening metabolic acidosis, respiratory distress, neurological impairment, hyperammonemia, and ketosis (PMID: 32679819). MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine (PMID: 23041189).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 17034-1-AP | Download protocol |
| IHC protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 17034-1-AP | Download protocol |
| IP protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 17034-1-AP | Download protocol |
| WB protocol for Methylmalonyl Coenzyme A mutase/MUT antibody 17034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Methylmalonyl Coenzyme A mutase/MUT polyclonal antibody (Cat# 17034-1-AP) has 9 citations across journals including Hepatology, EMBO Molecular Medicine, Inflammation Research, Cells, Gene Therapy, BBA Molecular Basis of Disease, American Journal of Pathology, Scientific Reports, and PLoS One. Research fields span methylmalonic acidemia, mitochondrial myopathy, ferroptosis, pulmonary fibrosis, genome editing, and metabolic homeostasis.
| Species | Application | Title |
|---|---|---|
Hepatology Promoterless, Nuclease-Free Genome Editing Confers a Growth Advantage for Corrected Hepatocytes in Mice With Methylmalonic Acidemia. | ||
EMBO Mol Med A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy | ||
Inflamm Res Methylmalonic acid as a ferroptosis-derived danger signal: activation of the PI3K-NF-κB pathway drives M1 macrophage polarization in renal ischemia-reperfusion injury. | ||
Cells Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA. | ||
Gene Ther First-in-human nuclease-free homologous recombination-dependent gene editing in pediatric patients with methylmalonic acidemia: results of a phase 1/2 study. | ||
Biochim Biophys Acta Mol Basis Dis ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis |
