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PIP4K2C Polyclonal antibody

PIP4K2C Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17077-1-AP

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

EC:2.7.1.149, Phosphatidylinositol 5-phosphate 4-kinase type II gamma, Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma, PI(5)P 4-kinase type II gamma, PIP4KII gamma

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 17077-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 17077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 17077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 17077-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 17077-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 17077-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 17077-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 17077-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 17077-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 17077-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse pancreas using 17077-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, PC-12 cells
Positive IHC detected in	human liver cancer tissue, human ovary cancer tissue, human tonsillitis tissue, mouse cerebellum tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17077-1-AP targets PIP4K2C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10989 Product name: Recombinant human PIP4K2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-421 aa of BC028596 Sequence: MASSSVPPATVSAATAGPGPGFGFASKTKKKHFVQQKVKVFRAADPLVGVFLWGVAHSINELSQVPPPVMLLPDDFKASSKIKVNNHLFHRENLPSHFKFKEYCPQVFRNLRDRFGIDDQDYLVSLTRNPPSESEGSDGRFLISYDRTLVIKEVSSEDIADMHSNLSNYHQYIVKCHGNTLLPQFLGMYRVSVDNEDSYMLVMRNMFSHRLPVHRKYDLKGSLVSREASDKEKVKELPTLRDMDFLNKNQKVYIGEEEKKIFLEKLKRDVEFLVQLKIMDYSLLLGIHDIIRGSEPEEEAPVREDESEVDGDCSLTGPPALVGSYGTSPEGIGGYIHSHRPLGPGEFESFIDVYAIRSAEGAPQKEVYFMGLIDILTQYDAKKKAAHAAKTVKHGAGAEISTVHPEQYAKRFLDFITNIFA Predict reactive species
Full Name	phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
Calculated Molecular Weight	421 aa, 47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	BC028596
Gene Symbol	PIP4K2C
Gene ID (NCBI)	79837
RRID	AB_2715526
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TBX8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIP4K2C is involved in several processes, including 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process; negative regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity; and positive regulation of autophagosome assembly. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism.

Protocols

Product Specific Protocols
IHC protocol for PIP4K2C antibody 17077-1-AP	Download protocol
WB protocol for PIP4K2C antibody 17077-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The PIP4K2C Polyclonal antibody (Cat# 17077-1-AP) has 9 total citations. These appear in journals including Cell Reports, Nature Communications, JBC, Phytomedicine, Journal of Advanced Research, Translational Oncology, Journal of Proteomics, Autophagy Reports, and eLife. Research fields span oncology (lung and breast cancer), ulcerative colitis, insulin signaling, protein interaction networks, proteomics, neurodegeneration (Huntington's disease), and cardiac reprogramming.

Species	Application	Title
human	WB	J Adv Res Orthosteric-allosteric dual inhibitors of PI5P4Kγ with potent antitumor activity in non-small cell lung cancer Authors - Liang Xiong View Article
human	WB,IF	Phytomedicine Traditional wisdom meets modern science: The protective role of sanhuang shu'ai decoction in ulcerative colitis models Authors - Nianchao Zhang View Article
human	WB	Cell Rep PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism. Authors - Diana G Wang KO Validated View Article
human	WB,IHC	Transl Oncol Downregulation of PIP4K2C inhibits the breast cancer cell proliferation, migration and invasion Authors - Yue Jin View Article
human	WB	J Biol Chem A proximity-labeling map of PI5P4K phosphoinositide kinases interaction networks. Authors - Alicia Llorente KO Validated View Article
		J Proteomics The in vitro effect of omentin-1 on the global proteome of granulosa cells from normal weight Large White and fat Meishan pigs. Authors - Karolina Pich View Article