Tested Applications
| Positive WB detected in | L02 cells, MCF-7 cells, Neuro-2a cells, HSC-T6 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17100-1-AP targets DGAT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10719 Product name: Recombinant human DGAT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 113-388 aa of BC015234 Sequence: DWNTPKKGGRRSQWVRNWAVWRYFRDYFPIQLVKTHNLLTTRNYIFGYHPHGIMGLGAFCNFSTEATEVSKKFPGIRPYLATLAGNFRMPVLREYLMSGGICPVSRDTIDYLLSKNGSGNAIIIVVGGAAESLSSMPGKNAVTLRNRKGFVKLALRHGADLVPIYSFGENEVYKQVIFEEGSWGRWVQKKFQKYIGFAPCIFHGRGLFSSDTWGLVPYSKPITTVVGEPITIPKLEHPTQQDIDLYHTMYMEALVKLFDKHKTKFGLPETEVLEVN Predict reactive species |
| Full Name | diacylglycerol O-acyltransferase homolog 2 (mouse) |
| Calculated Molecular Weight | 388 aa, 44 kDa |
| Observed Molecular Weight | 39~44kDa |
| GenBank Accession Number | BC015234 |
| Gene Symbol | DGAT2 |
| Gene ID (NCBI) | 84649 |
| RRID | AB_2918049 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PD7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Diacylglycerol acyltransferase 2 (DGAT2) is an integral membrane protein and major enzyme that catalyzes the final step of triacylglycerol (TG) synthesis in eukaryotes (PMID: 21680734). DGAT2 is present in mitochondria-associated membranes, specialized domains of the ER that are highly enriched in lipid synthetic enzymes (PMID: 19049983). DGAT2 isoforms (39 kDa, 44kDa) have major differences in acyl-CoA substrate specificity toward erucoyl-CoA.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DGAT2 antibody 17100-1-AP | Download protocol |
| WB protocol for DGAT2 antibody 17100-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DGAT2 Polyclonal antibody (Cat# 17100-1-AP) has 14 citations spanning journals including Nature Communications, Cell Death & Differentiation, Journal of Experimental & Clinical Cancer Research, Cell Reports, FEBS Journal, and PLoS ONE. Research fields covered include lipid metabolism, atherosclerosis, cancer, cardiac dysfunction, ferroptosis, myocardial ischemia-reperfusion, hepatic steatosis, and nonalcoholic fatty liver disease. Applications cited are primarily WB, with IHC and IF also represented.
| Species | Application | Title |
|---|---|---|
Nat Commun Sex-specific KDM6A-HNF4A-CREBH network controls lipoprotein cholesterol metabolism and atherosclerosis via epigenetic reprograming of hepatocytes. | ||
J Exp Clin Cancer Res Intracellular lipid droplets positively regulate the dormancy of colorectal cancer cells depending on ELF1-ACSL5 signaling | ||
Cell Death Differ Fatty acid channelling into triglycerides and oxylipins drives ferroptosis resistance during oncogenic BRAF-induced senescence.
| ||
Cell Death Differ Lipid overload-induced RTN3 activation leads to cardiac dysfunction by promoting lipid droplet biogenesis | ||
J Transl Med Notch3 enhances the synergistic effect of all-trans retinoic acid and calcipotriol in pancreatic stellate cell activation | ||
Cell Rep Targeting systemic and tumor metabolic balances with ketogenic diets enhance efficacy of therapy in FLT3-ITD acute myeloid leukemia. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars. One user reported excellent performance in human mesenchymal stem cells for Western blot (1/1000) and immunofluorescence (1/500), while the other noted crisp, clear bands in Western blot at a 1:1000 dilution. Overall, the antibody is well-received for producing strong, specific signals in both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Frauke (Verified Customer) (07-24-2026) | The antibody works very well in human mesenchymal stem cells (MSCs) in western blots (dilution 1/1000) and immunofluorescence (dilution 1/500).
|
Sai Sindhura (Verified Customer) (01-08-2026) | DGAT2 antibody gives crisp bands
|
