DUS1L Polyclonal antibody

DUS1L Polyclonal Antibody for WB, ELISA

Cat No. 17253-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DUS1, EC:1.3.1.88, PP3111, tRNA-dihydrouridine synthase 1-like, tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of Jurkat using 17253-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17253-1-AP (DUS1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 17253-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17253-1-AP (DUS1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17253-1-AP targets DUS1L in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10668 Product name: Recombinant human DUS1L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-406 aa of BC017081 Sequence: EVCPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQDEWDLLQRMILLAHEKLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPLSGAASWEHIKAVRKAVAIPVFANGNIQCLQDVERCLRDTGVQGVMSAEGNLHNPALFEGRSPAVWELAEEYLDIVREHPCPLSYVRAHLFKLWHHTLQVHQELREELAKVKTLEGIAAVSQELKLRCQEEISRQEGAKPTGDLPFHWICQPYIRPGPREGSKEKAGARSKRALEEEEGGTEVLSKNKQKKQLRNPHKTFDPSLKPKYAKCDQCGNPKGNRCVFSLCRGCCKKRASKETADCPGHGLLFKTKLEKSLAWKEAQPELQEPQPAAPGTPGGFSEVMGSALA Predict reactive species
Full Name	dihydrouridine synthase 1-like (S. cerevisiae)
Calculated Molecular Weight	473 aa, 53 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC017081
Gene Symbol	DUS1L
Gene ID (NCBI)	64118
RRID	AB_2094209
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6P1R4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DUS1L antibody 17253-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The DUS1L Polyclonal antibody (Cat# 17253-1-AP) has 1 total citation. The cited publication appeared in bioRxiv (2023), titled "Cell type-specific translational regulation by human DUS enzymes," which falls within the research fields of RNA biology and translational regulation. The antibody was used in Western blot (WB) on human samples.