Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17274-1-AP targets DZIP1L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11034 Product name: Recombinant human DZIP1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 418-767 aa of BC033308 Sequence: KAVDTEEDSPEEEMEDSQDEQHKVLAALRRNPTLLKHFRPILEDTLEEKLESMGIRKDAKGISIQTLRHLESLLRVQREQKARKFSEFLSLRGKLVKEVTSRAKERQENGAAVSQPDGQPSVKSQQSALVTREAQPKTRTLQVALPSTPAEPPPPTRQSHGSHGSSLTQVSAPAPHPGLHGPSSTPPSSGPGMSTPPFSSEEDSEGDRVQRVSLQPPKVPSRMVPRPKDDWDWSDTETSEENAQPPGQGSGTLVQSMVKNLEKQLEAPAKKPAGGVSLFFMPNAGPQRAATPGRKPQLSEDESDLEISSLEDLPLDLDQREKPKPLSRSKLPEKFGTGPQSSGQPRVPAW Predict reactive species |
| Full Name | DAZ interacting protein 1-like |
| Calculated Molecular Weight | 767 aa, 87 kDa |
| Observed Molecular Weight | 87 kDa |
| GenBank Accession Number | BC033308 |
| Gene Symbol | DZIP1L |
| Gene ID (NCBI) | 199221 |
| RRID | AB_3669266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DZIP1L, or DAZ interacting zinc finger protein 1-like, is a protein implicated in autosomal recessive polycystic kidney disease (ARPKD). DZIP1L has been shown to localize to centrioles and at the distal end of basal bodies, interacting with septin2, a protein that maintains the periciliary diffusion barrier at the ciliary transition zone (PMID: 28530676).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DZIP1L antibody 17274-1-AP | Download protocol |
| WB protocol for DZIP1L antibody 17274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DZIP1L Polyclonal antibody (Cat# 17274-1-AP) has 1 total citation. It was cited in Advanced Science (Impact Factor: 14.1) in a 2024 study by Huicheng Chen, which investigated how DZIP1L regulates ciliary protein entry by modulating ciliary transition fiber architecture and function in autosomal recessive polycystic kidney disease (ARPKD). The research field encompasses cilia biology and renal disease. The antibody was applied in immunofluorescence (IF) on human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers |
