Tested Applications
| Positive WB detected in | mouse spleen tissue, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17354-1-AP targets MKNK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10546 Product name: Recombinant human MKNK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-158 aa of BC018345 Sequence: MAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRWDSHFLLPPHPCRIHVRPGGLVRTVTVNE Predict reactive species |
| Full Name | MAP kinase interacting serine/threonine kinase 2 |
| Calculated Molecular Weight | 465 aa, 52 kDa |
| Observed Molecular Weight | 47-52 kDa |
| GenBank Accession Number | BC018345 |
| Gene Symbol | MKNK2 |
| Gene ID (NCBI) | 2872 |
| RRID | AB_2878395 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBH9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MKNK2 antibody 17354-1-AP | Download protocol |
| IHC protocol for MKNK2 antibody 17354-1-AP | Download protocol |
| WB protocol for MKNK2 antibody 17354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MKNK2 affinity-purified antibody (Cat# 17354-1-AP) has 7 total citations published in Clinical Sciences, Journal of Agricultural and Food Chemistry, Biochemical Pharmacology, Inflammation, Mediators of Inflammation, and Oncotarget. Research fields span insulin signaling in congenital heart disease, lipid metabolism, colorectal cancer metastasis, psoriasis, myasthenia gravis, renal cell carcinoma recurrence, and acute myeloid leukemia.
| Species | Application | Title |
|---|---|---|
Clin Sci (Lond) Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations. | ||
J Agric Food Chem Non-Sugar Sweetener Rubusoside Alleviates Lipid Metabolism Disorder In Vivo and In Vitro by Targeting PPARγ/α, Lgals3, and Mknk2 | ||
Biochem Pharmacol The role and mechanisms of the microRNA-1180-3p/MKNK2/CREB3 signaling axis in tumor progression following insufficient radiofrequency ablation of colorectal cancer liver metastasis | ||
Inflammation High Expression of miR-6785-5p in the Serum Exosomes of Psoriasis Patients Alleviates Psoriasis-Like Skin Damage by Interfering with the MNK2/p-eIF4E Axis in Keratinocytes
| ||
Mediators Inflamm Hsa_circ_0000313/miR-1224-3p/MKNK2 Axis Modulates CD4(+) T Cells by Activating p38 MAPK Signaling in Myasthenia Gravis. | ||
Oncotarget Impact of eIF4E phosphorylation at Ser209 via MNK2a on tumour recurrence after curative surgery in localized clear cell renal cell carcinoma. |
