Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17566-1-AP targets CEP41 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11764 Product name: Recombinant human TSGA14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC056162 Sequence: MSLRRHIGNPEYLMKRIPQNPRYQHIKSRLDTGNSMTKYTEKLEEIKKNYRYKKDELFKRLKVTTFAQLIIQVASLSDQTLEVTAEEIQRLEDNDSAASDPDAETTARTNGKGNPGEQSPSPEQFINNAGAGDSSRSTLQSVISGVGELDLDKGPVKKAEPHTKDKPYPDCPFLLLDVRDRDSYQQCHIVGAYSYPIATLSRTMNPYSNDILEYKNAHGKIIILYDDDERLASQAATTMCERGFENLFMLSGGRLNQANSSGRESKVPGARSAQNLPGGGPASHSNPRSLSSGHLQGKPWK Predict reactive species |
| Full Name | testis specific, 14 |
| Calculated Molecular Weight | 301aa,33 kDa; 373aa,41 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC056162 |
| Gene Symbol | TSGA14 |
| Gene ID (NCBI) | 95681 |
| RRID | AB_2878417 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYV8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TSGA14 (testis-specific Gene A14), also known as CEP41 (centrosomal protein of 41 kDa) , is a centrosomal protein identified in a mass-spectrometry-based proteomic analysis of human centrosomes. Mutations in the TSGA14 gene have been identified in families with autism spectrum disorders. (PMID:21438139)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CEP41 antibody 17566-1-AP | Download protocol |
| WB protocol for CEP41 antibody 17566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CEP41 Polyclonal antibody (Cat# 17566-1-AP) has 2 total citations. These appear in Molecular Psychiatry and Journal of Cell Science. The cited research spans neurodevelopment (autism spectrum disorder–related mutations in CEP41 affecting cortical organoid development) and cell biology (CEP41's role in microtubule assembly and cell proliferation). Applications used in these studies include immunofluorescence and Western blot.
| Species | Application | Title |
|---|---|---|
Mol Psychiatry ASD mutations in the ciliary gene CEP41 impact development of projection neurons and interneurons in a human cortical organoid model. | ||
J Cell Sci CEP41, a ciliopathy-linked centrosomal protein, regulates microtubule assembly and cell proliferation
|
