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PAAF1 Polyclonal antibody

PAAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17650-1-AP
Publications (2)
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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PAAF, Proteasomal ATPase-associated factor 1, Protein G-16, Rpn14, WD repeat-containing protein 71

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of Jurkat cells using PAAF1 Polyclonal antibody (17650-1-AP)

    WB analysis of Jurkat using 17650-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of mouse kidney using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of mouse brain using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of mouse brain using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of mouse kidney using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human testis tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human testis using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human testis tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human testis using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human kidney tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human kidney using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human brain using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human kidney tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human kidney using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human brain tissue using PAAF1 Polyclonal antibody (17650-1-AP)

    IHC staining of human brain using 17650-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PAAF1 Polyclonal antibody (17650-1-AP)

    IF Staining of U2OS using 17650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAAF1 antibody (17650-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse kidney tissue, human brain tissue, human kidney tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17650-1-AP targets PAAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11892

Product name: Recombinant human PAAF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-375 aa of BC028628

Sequence: MLVPCFLYSLQNRKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHISCPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCCRFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSACLGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGSDAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQDLDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL

Predict reactive species
Full Name proteasomal ATPase-associated factor 1
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight40-43 kDa
GenBank Accession NumberBC028628
Gene Symbol PAAF1
Gene ID (NCBI) 80227
RRIDAB_2878424
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRP4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for PAAF1 antibody 17650-1-APDownload protocol
IHC protocol for PAAF1 antibody 17650-1-APDownload protocol
WB protocol for PAAF1 antibody 17650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The PAAF1 polyclonal antibody (Cat# 17650-1-AP) has 2 citations. These appear in American Journal of Cancer Research (2019) and eLife (2023). Both studies fall within the field of cancer research—one investigating paclitaxel resistance in esophageal squamous cell carcinoma via CRISPR screening, and the other examining proteomic signatures of T1 colorectal cancer metastasis to lymph nodes. The antibody was used for WB and IHC applications in human samples, respectively.

Automatically generated from the 2 publication citations by Able AI.
SpeciesApplicationTitle
humanWB

Am J Cancer Res

PMID: 31285951

Authors - Wen-Si Zhao
View Article
humanIHC

Elife

Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes

Authors - Aojia Zhuang
View Article
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