Tested Applications
| Positive WB detected in | Jurkat cells, U-937 cells, Ramos cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17699-1-AP targets ICAM3/CD50 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11955 Product name: Recombinant human ICAM3 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 200-547 aa of BC058903 Sequence: GLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSHFVPVFVAVLLTLGVVTIVLALMYVFREHQRSGSYHVREESTYLPLTSMQPTEAMGEEPSRAE Predict reactive species |
| Full Name | intercellular adhesion molecule 3 |
| Calculated Molecular Weight | 547 aa, 60 kDa |
| Observed Molecular Weight | 110-160 kDa, 60 kDa |
| GenBank Accession Number | BC058903 |
| Gene Symbol | ICAM3/CD50 |
| Gene ID (NCBI) | 3385 |
| RRID | AB_2248763 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32942 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ICAM3 (Intercellular adhesion molecule 3) is also named as CD50, CDw50 and ICAM-R. ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. ICAM3, being expressed constitutively on T cells and other leukocytes, is a potent signalling molecule and a major ligand in the initiation of T cell-mediated immune responses (PMID: 1448173, PMID: 11812993). ICAM3 is the natural ligand for both DC-SIGN and L-SIGN (PMID: 17570115). ICAM3 is abundantly and constitutively expressed in all leukocytes and is probably the most important ligand for LFA-1 in initiating immune responses, and ICAM3 is a typical type I transmembrane glycoprotein (PMID: 39991572). The promoter region of ICAM3 can be bound by co-transcription factors such as ETs and C/EBP, promoting macrophage maturation as well as monocyte extravasation (PMID: 22479382). ICAM3 is widely expressed not only in lymphocytes such as T and B cells but also in many endothelial cells and dendritic cells (PMID: 39991572).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ICAM3/CD50 antibody 17699-1-AP | Download protocol |
| IP protocol for ICAM3/CD50 antibody 17699-1-AP | Download protocol |
| WB protocol for ICAM3/CD50 antibody 17699-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One user reviewed this antibody for Western Blot on Jurkat cells at a 1/1000 dilution, giving it a 5-star rating. They confirmed that the antibody works at the dilution specified by the supplier, indicating reliable performance out of the box.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jean-Baptiste (Verified Customer) (09-13-2026) | The antibody works at the dilution specified by the supplier in a Western blot
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