Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17718-1-AP targets METTL4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12093 Product name: Recombinant human METTL4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 125-472 aa of BC111020 Sequence: ISISIIGKKRKRCVVFNQGELDAMEYHTKIRELILDGSLQLIQEGLKSGFLYPLFEKQDKGSKPITLPLDACSLSELCEMAKHLPSLNEMEHQTLQLVEEDTSVTEQDLFLRVVENNSSFTKVITLMGQKYLLPPKSSFLLSDISCMQPLLNYRKTFDVIVIDPPWQNKSVKRSNRYSYLSPLQIKQIPIPKLAAPNCLLVTWVTNRQKHLRFIKEELYPSWSVEVVAEWHWVKITNSGEFVFPLDSPHKKPYEGLILGRVQEKTALPLRNADVNVLPIPDHKLIVSVPCTLHSHKPPLAEVLKDYIKPDGEYLELFARNLQPGWTSWGNEVLKFQHVDYFIALESGS Predict reactive species |
| Full Name | methyltransferase like 4 |
| Calculated Molecular Weight | 472 aa, 54 kDa |
| GenBank Accession Number | BC111020 |
| Gene Symbol | METTL4 |
| Gene ID (NCBI) | 64863 |
| RRID | AB_2142047 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3J2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Methyltransferase like protein 4 (METTL4) is a internal m6Am methyltransferase, which mediates N6-methylation of Am30 on U2 snRNA in the context of an AAG motif in vivo and in vitro. METTL4 belongs to the MT-A70-like family, and like its paralogs METTL3/14, METTL4 is conserved from yeast to human.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for METTL4 antibody 17718-1-AP | Download protocol |
| IHC protocol for METTL4 antibody 17718-1-AP | Download protocol |
| WB protocol for METTL4 antibody 17718-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The METTL4 Polyclonal antibody (Cat# 17718-1-AP) has 4 citations published in J Transl Med, EMBO J, Future Oncol, and Am J Cancer Res. The associated research fields include epigenetic signatures in tumor progression, brown adipose tissue formation via RNA methyltransferase stabilization, hepatocellular carcinoma recurrence biomarkers, and m6A RNA modification in cancer chemoresistance. Applications cited include WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Transl Med A machine learning-based epigenetic signature reveals YTHDC1 stabilizes POU5F1 to oppose tumor progression. | ||
EMBO J PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase. | ||
Future Oncol METTL4 and METTL5 as biomarkers for recurrence-free survival in hepatocellular carcinoma patients | ||
Am J Cancer Res Binding of RNA m6A by IGF2BP3 triggers chemoresistance of HCT8 cells via upregulation of ABCB1. |
