Tested Applications
| Positive WB detected in | HEK-293 cells, K-562 cells, HepG2 cells, human placenta tissue, mouse kidney tissue, HuH-7 cells, COS-7 cells |
| Positive IHC detected in | human placenta tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17804-1-AP targets Calumenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.
| Tested Reactivity | human, mouse, monkey |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12073 Product name: Recombinant human CALU protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-315 aa of BC013383 Sequence: MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF Predict reactive species |
| Full Name | calumenin |
| Calculated Molecular Weight | 315 aa, 37 kDa |
| Observed Molecular Weight | 47 kDa, 57 kDa |
| GenBank Accession Number | BC013383 |
| Gene Symbol | Calumenin |
| Gene ID (NCBI) | 813 |
| RRID | AB_2069964 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43852 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Calumenin antibody 17804-1-AP | Download protocol |
| IHC protocol for Calumenin antibody 17804-1-AP | Download protocol |
| WB protocol for Calumenin antibody 17804-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Calumenin (CALU) antibody (Cat# 17804-1-AP) has 6 citations across journals including Cell Research, Oncogene, Respiratory Research, Journal of Inflammation Research, Cancer Medicine, and Cell and Tissue Research. Research fields span DNA damage and apoptosis, ERK signaling and cell migration, lung adenocarcinoma progression, EMT in triple-negative breast cancer, gastric cancer prognosis, and hormone-regulated PKA activity in porcine oviductal epithelial cells.
| Species | Application | Title |
|---|---|---|
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Oncogene Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis.
| ||
Respir Res CALU promotes lung adenocarcinoma progression by enhancing cell proliferation, migration and invasion | ||
J Inflamm Res Pan-Cancer Screening and Validation of CALU's Role in EMT Regulation and Tumor Microenvironment in Triple-Negative Breast Cancer | ||
Cancer Med Five EMT-related genes signature predicts overall survival and immune environment in microsatellite instability-high gastric cancer. | ||
