RANBP3L Polyclonal antibody

RANBP3L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17875-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

RAN binding protein 3 like, RANBP3L

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 17875-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 17875-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 17875-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 17875-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 17875-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 17875-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 17875-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells
Positive IHC detected in	mouse kidney tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17875-1-AP targets RANBP3L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11642 Product name: Recombinant human RANBP3L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 110-465 aa of BC047660 Sequence: SAEQGPVKHSKHVIRPAILQLPQARSCAKVRKTFGHKALESCKTKEKTNNKISEGNSYLLSENLSRARISVQLSTNQDFLGATSVGCQPNEDKCSFKSCSSNFVFGENMVERVLGTQKLTQPQLENDSYAKEKPFKSIPKFPVNFLSSRTDSIKNTSLIESAAAFSSQPSRKCLLEKIDVITGEETEHNVLKINCKLFIFNKTTQSWIERGRGTLRLNDTASTDCGTLQSRLIMRNQGSLRLILNSKLWAQMKIQRANHKNVRITATDLEDYSIKIFLIQASAQDTAYLYAAIHHRLVALQSFNKQRDVNQAESLSETAQQLNCESCDENEDDFIQVTKNGSDPSSWTHRQSVACS Predict reactive species
Full Name	RAN binding protein 3-like
Calculated Molecular Weight	465 aa, 52 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC047660
Gene Symbol	RANBP3L
Gene ID (NCBI)	202151
RRID	AB_2284894
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86VV4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for RANBP3L antibody 17875-1-AP	Download protocol
WB protocol for RANBP3L antibody 17875-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The RANBP3L affinity purification antibody (Cat# 17875-1-AP) has 2 citations. These appear in Cell Mol Biol Lett (IF 12.2) and Cancer Rep (IF 2.5). The cited studies focus on cancer research: one investigates tiRNA-Met's role in inhibiting triple-negative breast cancer progression via RANBP3L/SNRPA interaction (IF application), while the other examines RANBP3L's significance in pan-cancer and hepatocellular carcinoma (WB application). Both publications highlight RANBP3L's involvement in malignant tumor biology.

Species	Application	Title
	IF	Cell Mol Biol Lett The novel tRNA-derived fragment, tiRNA-Met, inhibits the malignant progression of triple-negative breast cancer by regulating RANBP3L via a targeted interaction with SNRPA Authors - Jingjing Lu View Article
human	WB	Cancer Rep (Hoboken) The Role of RANBP3L in Pan-Cancer With Its Significance in Hepatocellular Carcinoma. Authors - Beini Cen View Article