Tested Applications
| Positive WB detected in | MDA-MB-231 cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17882-1-AP targets LIN9 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11889 Product name: Recombinant human LIN9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-296 aa of BC045625 Sequence: MHRGGQPLKKRRGSFKMAELDQLPDESSSAKALVSLKEGSLSNTWNEKYSSLQKTPVWKGRNTSSAVEMPFRNSKRSRLFSDEDDRQINTRSPKRNQRVAMVPQKFTATMSTPDKKASQKIGFRLRNLLKLPKAHKWCIYEWFYSNIDKPLFEGDNDFCVCLKESFPNLKTRKLTRVEWGKIRRLMGKPRRCSSAFFEEERSALKQKRQKIRLLQQRKVADVSQFKDLPDEIPLPLVIGTKVTARLRGVHDGLFTGQIDAVDTLNATYRVTFDRTGLGTHTIPDYEVLSNEPHETM Predict reactive species |
| Full Name | lin-9 homolog (C. elegans) |
| Calculated Molecular Weight | 558 aa, 64 kDa |
| Observed Molecular Weight | 55-65 kDa |
| GenBank Accession Number | BC045625 |
| Gene Symbol | LIN9 |
| Gene ID (NCBI) | 286826 |
| RRID | AB_10859819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TKA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LIN9, also named as BARA or TGS, is a 542 amino acid protein, which belongs to the lin9 family. LIN9 localizes in the nucleus and is expressed in thymus and testis. LIN9 acts as a tumor suppressor and inhibits DNA synthesis. LIN9 plays a role in the expression of genes, which required for the G1/S transition.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for LIN9 antibody 17882-1-AP | Download protocol |
| WB protocol for LIN9 antibody 17882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LIN9 Polyclonal Antibody (Cat# 17882-1-AP) has 7 citations published in Cell Death Dis, Cell Death Discov, J Transl Med, Sci China Life Sci, EMBO Mol Med, Int J Oncol, and FEBS Open Bio. Research fields span cancer biology (non-small cell lung cancer, triple-negative breast cancer, hepatocellular carcinoma), vascular restenosis, cell cycle regulation, intestinal stem cell homeostasis, and cellular senescence. Applications cited include WB, IF, and CoIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis | ||
Cell Death Discov p21 Regulates Wnt-Notch balance via DREAM/MMB/Rb-E2F1 and maintains intestinal stem cell homeostasis | ||
J Transl Med Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation
| ||
EMBO Mol Med Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple-negative breast cancer | ||
Int J Oncol Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9 |
