Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17889-1-AP targets SLTM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12060 Product name: Recombinant human SLTM protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 154-502 aa of BC046119 Sequence: RERIRIEQERRKEAERIAREREELRRQQQQLRYEQEKRNSLKRPRDVDHRRDDPYWSENKKLSLDTDARFGHGSDYSRQQNRFNDFDHRERGRFPESSAVQSSSFERRDRFVGQSEGKKARPTARREDPSFERYPKNFSDSRRNEPPPPRNELRESDRREVRGERDERRTVIIHDRPDITHPRHPREAGPNPSRPTSWKSEGSMSTDKRETRVERPERSGREVSGHSVRGAPPGNRSSASGYGSREGDRGVITDRGGGSQHYPEERHVVERHGRDTSGPRKEWHGPPSQGPSYHDTRRMGDGRAGAGMITQHSSNASPINRIVQISGNSMPRGSGSGFKPFKGGPPRRF Predict reactive species |
| Full Name | SAFB-like, transcription modulator |
| Calculated Molecular Weight | 478aa,57 kDa; 1034aa,117 kDa |
| Observed Molecular Weight | 135-140 kDa |
| GenBank Accession Number | BC046119 |
| Gene Symbol | SLTM |
| Gene ID (NCBI) | 79811 |
| RRID | AB_2192729 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NWH9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLTM, also named as Modulator of estrogen-induced transcription, is a 1034 amino acid protein, which localizes in the nucleus. When it is overexpressed, SLTM acts as a general inhibitor of transcription that eventually leads to apoptosis. The calculated molecular weight of SLTM is 117 kDa, but modified SLTM is about 135-140 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SLTM antibody 17889-1-AP | Download protocol |
| IHC protocol for SLTM antibody 17889-1-AP | Download protocol |
| WB protocol for SLTM antibody 17889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLTM polyclonal antibody (Cat# 17889-1-AP) has 1 citation. It appears in the Journal of Animal Science and Biotechnology, in a study on bovine testicular tissue development using spatial transcriptomics. The associated research field is animal reproductive biology, specifically examining testicular tissue development in cattle via spatial transcriptomic analysis. The antibody was applied in immunofluorescence (IF) for this bovine study.
| Species | Application | Title |
|---|---|---|
J Anim Sci Biotechnol Dissecting the development of bovine testicular tissue using spatial transcriptomics. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot applications. One user reported specific Sltm detection in U2OS cells at a 1:2000 dilution, while the other described it as an "excellent antibody" when used on RKO cells at 1:3000 dilution. Both reviews were from the same lot and highlight strong specificity and reliable performance in human cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tsimafei (Verified Customer) (08-30-2025) | Specific Sltm detection in human cells
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Aleksandr (Verified Customer) (07-29-2025) | Excellent antibody!
 |
