Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human lung cancer tissue, human malignant melanoma tissue, mouse testis tissue, rat kidney tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18038-1-AP targets SMURF2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12666 Product name: Recombinant human SMURF2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 390-748 aa of BC093876 Sequence: AGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE Predict reactive species |
| Full Name | SMAD specific E3 ubiquitin protein ligase 2 |
| Calculated Molecular Weight | 748 aa, 86 kDa |
| Observed Molecular Weight | 86-100 kDa |
| GenBank Accession Number | BC093876 |
| Gene Symbol | SMURF2 |
| Gene ID (NCBI) | 64750 |
| RRID | AB_3085549 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAU4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210). SMURF2 can be detected 100 kDa by poly-sumoylated (PMID:26679521).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMURF2 antibody 18038-1-AP | Download protocol |
| IP protocol for SMURF2 antibody 18038-1-AP | Download protocol |
| WB protocol for SMURF2 antibody 18038-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SMURF2 antibody (Cat# 18038-1-AP) has 16 citations across journals including Mol Cell, Adv Sci, Cancer Lett, Cell Rep, Phytomedicine, Acta Pharmacol Sin, Front Immunol, Int J Mol Sci, Cancer Sci, and others. Research fields span cancer biology (ferroptosis, pancreatic/breast/ovarian/bladder/nasopharyngeal carcinoma), inflammation and inflammasome signaling, cardiac hypertrophy, myocardial ischemia-reperfusion injury, Alzheimer's disease, psoriasis, PCOS, neuropathic pain, and autophagy regulation.
| Species | Application | Title |
|---|---|---|
Mol Cell SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation
| ||
Adv Sci (Weinh) Targeting Intratumoral Copper Inhibits Tumor Progression via p62-Mediated EZH2 Degradation and Potentiates Anti-PD-1 Immunotherapy in Oral Squamous Cell Carcinoma
| ||
Adv Sci (Weinh) Neddylation Targets and Stabilizes NLRP3 to Augment Inflammasome-Mediated Colitis and Mood Disorder. | ||
Cancer Lett UBE2C promotes pancreatic tumorigenesis by KRAS stabilization via APC/CCDH1-mediated WDR76 degradation | ||
Phytomedicine Jiao-tai-wan and its component coptisine attenuate PCOS by regulating mitochondrial cholesterol import through suppression of SIRT1 ubiquitination. | ||
Acta Pharmacol Sin SMURF2 inhibition attenuates cardiac hypertrophy through blocking ubiquitination degradation of AXIN1
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