Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18129-1-AP targets GCOM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12708 Product name: Recombinant human GCOM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-466 aa of BC101645 Sequence: MYGDYDEMRQKIRQLTQELSVSHAQQEYLENHIQTQSSALDRFNAMNSALASDSIGLQKTLVDVTLENSNIKDQIRNLQQTYEASMDKLREKQRQLEVAQVENQLLKMKVESSQEANAEVMREMTKKLYSQYEEKLQEEQRKHSAEKEALLEETNSFLKAIEEANKKMQAAEISLEEKDQRIGELDRLIERMEKERHQLQLQLLEHETEMSGELTDSDKERYQQLEEASASLRERIRHLDDMVHCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEKISFLEGENNELQSRLDYLTETQAKTEVETREIGVGCDLLPSQTGRTREIVMPSRNYTPYTRVLELTMKKTLT Predict reactive species |
| Full Name | GRINL1A complex locus |
| Calculated Molecular Weight | 466 aa, 54 kDa |
| Observed Molecular Weight | 64 kDa, 44-54 kDa |
| GenBank Accession Number | BC101645 |
| Gene Symbol | GCOM1 |
| Gene ID (NCBI) | 145781 |
| RRID | AB_2232101 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0CAP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GCOM1, also named as Myozap, Gup and Gcom, belongs to the GCOM1 family. It plays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF. GCOM1 has 11 isoforms with MW 44-54kd and 64kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GCOM1 antibody 18129-1-AP | Download protocol |
| IHC protocol for GCOM1 antibody 18129-1-AP | Download protocol |
| WB protocol for GCOM1 antibody 18129-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GCOM1 Polyclonal antibody (Cat# 18129-1-AP) has 1 citation to date. It was cited in Molecular Cell (2024), a study by Cacioppo et al. investigating how the CRL3ARMC5 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription. The antibody was applied in Western Blot (WB) on human samples. The associated research field is transcriptional regulation and RNA biology.
| Species | Application | Title |
|---|---|---|
Mol Cell CRL3ARMC5 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription |
