Tested Applications
| Positive WB detected in | mouse uterus tissue, rat uterus tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18158-1-AP targets MYL6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12964 Product name: Recombinant human MYL6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC006781 Sequence: MCDFTEDQTAEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVAGHEDSNGCINYEAFVRHILSG Predict reactive species |
| Full Name | myosin, light chain 6, alkali, smooth muscle and non-muscle |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 15-20 kDa |
| GenBank Accession Number | BC006781 |
| Gene Symbol | MYL6 |
| Gene ID (NCBI) | 4637 |
| RRID | AB_3085557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60660 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYL6 antibody 18158-1-AP | Download protocol |
| IHC protocol for MYL6 antibody 18158-1-AP | Download protocol |
| WB protocol for MYL6 antibody 18158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this antibody is highly favorable, rated 5 stars. The user reported using it at a 1:3000 dilution for Western Blot on mouse endothelial cells, rat smooth muscle cells, and HEK293 cells. They described it as a good antibody that performs well across a wide range of experiments, indicating reliable performance and broad applicability. Overall, the feedback is positive, with no reported issues regarding specificity, sensitivity, or ease of use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaurav (Verified Customer) (11-14-2025) | Good antibody which works also in wide range of experiments.
|
