Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18178-1-AP targets RBM24 in WB, IHC, IF-P, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12871 Product name: Recombinant human RBM24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC104808 Sequence: MYVCLCVSVAKVTMADRAAAERACKDPNPIIDGRKANVNLAYLGAKPRIMQPGFAFGVQQLHPALIQRPFGIPAHYVYPQAFVQPGVVIPHVQPTAAAASTTPYIDYTGAAYAQYSAAAAAAAAAAAYDQYPYAASPAAAGYVTAGGYGYAVQQPITAAAPGTAAAAAAAAAAAAAFGQYQPQQLQTDRMQ Predict reactive species |
| Full Name | RNA binding motif protein 24 |
| Calculated Molecular Weight | 191 aa, 20 kDa |
| Observed Molecular Weight | 18-25 kDa |
| GenBank Accession Number | BC104808 |
| Gene Symbol | RBM24 |
| Gene ID (NCBI) | 221662 |
| RRID | AB_2878513 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BX46 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development, they exhibit temporally and spatially regulated expression patterns and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs (PMID: 22510371). Their expression is tissue-specific, and their crucial influence on the protein setup, especially in muscle cells, has become recognized recently (PMID: 21982546). RBM24 is one RNA-binding protein that is fundamental in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility. It may thus represent a novel pathway to cardiomyopathy (PMID: 20338237).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBM24 antibody 18178-1-AP | Download protocol |
| IHC protocol for RBM24 antibody 18178-1-AP | Download protocol |
| WB protocol for RBM24 antibody 18178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-RBM24 antibody (Cat# 18178-1-AP) has 14 citations spanning journals including Cell Death & Differentiation, PNAS, eLife, Development, Scientific Reports, and International Journal of Biological Macromolecules. Research fields cover cancer biology (ovarian, lung, colorectal, thyroid carcinoma), cochlear hair cell development and hearing, myogenic differentiation, RNA splicing regulation, cuproptosis, and neurovascular disease. Applications include WB, IF, IHC, and RIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Differ Tropomyosin1 isoforms underlie epithelial to mesenchymal plasticity, metastatic dissemination, and resistance to chemotherapy in high-grade serous ovarian cancer | ||
Proc Natl Acad Sci U S A Alternative splicing of the Rbm24 gene is essential for cochlear hair cell stereocilia integrity and hearing function in mice
| ||
Int J Biol Macromol Rbm24 modulates neuronal RNA splicing to restrict cognitive dysfunction | ||
J Mol Med (Berl) Mechanism of METTL14-mediated m6A modification in non-small cell lung cancer cell resistance to cisplatin | ||
Sci Rep Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration.
| ||
J Cell Physiol RBM24 is required for mouse hair cell development through regulating pre-mRNA alternative splicing and mRNA stability |
