Tested Applications
| Positive WB detected in | MCF-7 cells, mouse liver tissue, human heart tissue, mouse skeletal muscle tissue, U2OS cells, A549 cells, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18476-1-AP targets PPP1R14B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13373 Product name: Recombinant human PPP1R14B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC014522 Sequence: MADSGTAGGAALAAPAPGPGSGGPGPRVYFQSPPGAAGEGPGGADDEGPVRRQGKVTVKYDRKELRKRLNLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDARAARVKELLVDCYKPTEAFISGLLDKIRGMQKLSTPQKK Predict reactive species |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 14B |
| Calculated Molecular Weight | 147 aa, 16 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC014522 |
| Gene Symbol | PPP1R14B |
| Gene ID (NCBI) | 26472 |
| RRID | AB_10598019 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96C90 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP1R14B antibody 18476-1-AP | Download protocol |
| WB protocol for PPP1R14B antibody 18476-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 18476-1-AP has a total of 4 citations. These appear in Immunity, Biochim Biophys Acta Mol Basis Dis, Cell Signal, and J Cell Mol Med. The associated research fields include fibrosis development in nonhematopoietic cells, cervical cancer characterization via lysine lactylation, prostate cancer progression through the JAK2/STAT3/AR pathway, and endometrial carcinoma diagnosis and prognosis. Applications cited include WB and IHC in both human and mouse species.
| Species | Application | Title |
|---|---|---|
Immunity Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis. | ||
Biochim Biophys Acta Mol Basis Dis Lysine lactylation-based insight to understanding the characterization of cervical cancer | ||
Cell Signal PPP1R14B promotes the progression of prostate cancer by regulating the JAK2/STAT3/AR pathway and alters the sensitivity and resistance to enzalutamide. | ||
J Cell Mol Med PPP1R14B is a diagnostic prognostic marker in patients with uterine corpus endometrial carcinoma |
