FUT10 Polyclonal antibody

FUT10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18660-1-AP

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Alpha-(1,3)-fucosyltransferase 10, EC:2.4.1.-, Fuc TX, FucT X, FucTX

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of HeLa using 18660-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18660-1-AP (FUT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 18660-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18660-1-AP (FUT10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 18660-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18660-1-AP (FUT10 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 18660-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18660-1-AP (FUT10 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 18660-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18660-1-AP (FUT10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 18660-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18660-1-AP (FUT10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IHC detected in	human testis tissue, mouse kidney tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18660-1-AP targets FUT10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13415 Product name: Recombinant human FUT10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 172-521 aa of BC063462 Sequence: NIDSLPLPRKAHHDWAVFHEESPKNNYKLFHKPVITLFNYTATFSRHSHLPLTTQYLESIEVLKSLRYLVPLQSKNKLRKRLAPLVYVQSDCDPPSDRDSYVRELMTYIEVDSYGECLRNKDLPQQLKNPASMDADGFYRIIAQYKFILAFENAVCDDYITEKFWRPLKLGVVPVYYGSPSITDWLPSNKSAILVSEFSHPRELASYIRRLDSDDRLYEAYVEWKLKGEISNQRLLTAVREPKWGVQDVNQDNYIDAFECMVCTKVWANIRLQEKGLPPKRWEAEDTHLSCPEPTVFAFSPLRTPPLSSLREMWISSFEQSKKEAQALRWLVDRNQNFSSQEFWGLVFKD Predict reactive species
Full Name	fucosyltransferase 10 (alpha (1,3) fucosyltransferase)
Calculated Molecular Weight	521 aa, 61 kDa
Observed Molecular Weight	41-48 kDa
GenBank Accession Number	BC063462
Gene Symbol	FUT10
Gene ID (NCBI)	84750
RRID	AB_10641997
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6P4F1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for FUT10 antibody 18660-1-AP	Download protocol
WB protocol for FUT10 antibody 18660-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The FUT10 polyclonal antibody (Cat# 18660-1-AP) has 3 citations published in Nature Chemical Biology, Arthritis Research & Therapy, and eLife. The associated research fields include glycobiology (protein O-fucosylation of EMI domains), rheumatology/immunology (α-1,3 fucosylation promoting TNFR1–TNF-α binding in osteoarthritic cartilage destruction), and neuroscience/RNA biology (NMD factor UPF3B in olfactory sensory neurons).

Species	Application	Title
human	IF	Nat Chem Biol FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains Authors - Huilin Hao View Article
human	WB	Arthritis Res Ther Elevation of α-1,3 fucosylation promotes the binding ability of TNFR1 to TNF-α and contributes to osteoarthritic cartilage destruction and apoptosis. Authors - Hanjie Yu View Article
mouse	IF	Elife The role of the NMD factor UPF3B in olfactory sensory neurons. Authors - Kun Tan View Article