Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18830-1-AP targets 1A6/DRIM in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | UTP20, small subunit (SSU) processome component, homolog (yeast) |
| Calculated Molecular Weight | 318 kDa |
| Observed Molecular Weight | 310 kDa |
| GenBank Accession Number | NM_014503 |
| Gene Symbol | UTP20 |
| Gene ID (NCBI) | 27340 |
| RRID | AB_10597541 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75691 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
1A6/DRIM has been identified as UTP20, a small subunit processome component, functioning in 18S rRNA processing. 1A6/DRIM was originally identified from the malignant derivative of an immortalized gastric epithelial cell line [1] and was found to be down-regulated in metastatic breast cancer cells (PMID: 21151873, 17498821).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for 1A6/DRIM antibody 18830-1-AP | Download protocol |
| IP protocol for 1A6/DRIM antibody 18830-1-AP | Download protocol |
| WB protocol for 1A6/DRIM antibody 18830-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The 1A6/DRIM (UTP20) polyclonal antibody (Cat# 18830-1-AP) has 2 citations. They appear in Frontiers in Immunology (2025) and Cell Signaling (2026). Both studies used the antibody for Western blot in human samples. The research fields cover glioma—examining ribosome biogenesis gene signatures, prognosis, and immune landscape—and colon cancer, identifying HMMR as a prognostic marker and potential therapeutic target.
| Species | Application | Title |
|---|---|---|
Front Immunol Ribosome biogenesis-related gene signature predicts prognosis and immune landscape in glioma and identifies UTP20 as a therapeutic target.
| ||
Cell Signal HMMR is a novel prognostic marker and a potential therapeutic target for colon cancer.
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