Tested Applications
| Positive WB detected in | mouse skin tissue |
| Positive IHC detected in | human colon tissue, human stomach cancer tissue, human appendicitis tissue, rat lung tissue, rat stomach tissue, mouse liver tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18837-1-AP targets HMCN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | hemicentin 1 |
| Calculated Molecular Weight | 613 kDa, 600 kDa, 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_031935 |
| Gene Symbol | HMCN1 |
| Gene ID (NCBI) | 83872 |
| RRID | AB_2878563 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RW7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-976 amino acids of human HMCN1 can recognize all three isoforms of HMCN1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HMCN1 antibody 18837-1-AP | Download protocol |
| IHC protocol for HMCN1 antibody 18837-1-AP | Download protocol |
| WB protocol for HMCN1 antibody 18837-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HMCN1 Polyclonal antibody (Cat# 18837-1-AP) has 4 total citations published in J Exp Med, Front Pharmacol, Front Cell Dev Biol, and PLoS Pathog. The associated research fields include epidermolysis bullosa genetics, myocardial ischemia-reperfusion injury, tooth root development and mesenchymal cell differentiation, and bovine leukemia virus replication and oncogenesis.
| Species | Application | Title |
|---|---|---|
Front Pharmacol Artemisinin relieves myocardial ischemia-reperfusion injury via modulating miR-29b-3p and hemicentin 1 | ||
Front Cell Dev Biol Hemicentin-1 is an essential extracellular matrix component during tooth root formation by promoting mesenchymal cells differentiation | ||
PLoS Pathog Reduction of antisense transcription affects bovine leukemia virus replication and oncogenesis |
