Tested Applications
| Positive WB detected in | MCF-7 cells, cells, SW480 cells, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18901-1-AP targets TAF3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13536 Product name: Recombinant human TAF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 443-721 aa of BC024661 Sequence: TKSGSTPLPLSGGTSSSDNSWTMDASIDEVVRKAKLGTPSNMPPNFPYISSPSVSPPTPEPLHKVYEEKTKLPSSVEVKKKLKKELKTKMKKKEKQRDREREKDKNKDKSKEKDKVKEKEKDKETGRETKYPWKEFLKEEEADPYKFKIKEFEDVDPKVKLKDGLVRKEKEKHKDKKKDREKGKKDKDKREKEKVKDKGREDKMKAPAPPLVLPPKELALPLFSPATASRVPAMLPSLLPVLPEKLFEEKEKVKEKEKKKDKKEKKKKKEKEKEKKEK Predict reactive species |
| Full Name | TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa |
| Calculated Molecular Weight | 929 aa, 104 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC024661 |
| Gene Symbol | TAF3 |
| Gene ID (NCBI) | 83860 |
| RRID | AB_2878567 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VWG9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TAF3, also named as TAF140, is a 140 kDa subunit of Transcription initiation factor TFIID. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF3 has some isoforms with 727aa (~80 kDa), 622aa and 929aa (105 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF3 antibody 18901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TAF3 Polyclonal antibody (Cat# 18901-1-AP) has 2 citations. One appears in mBio (2014), studying epigenetic factors regulating herpesviral gene expression via RNA interference screening. The other is in Oncology Letters (2025), identifying a FOXO3-associated prognostic model in hepatocellular carcinoma. Both studies used the antibody for Western blot in human samples, spanning research fields of viral epigenetics and cancer prognostic biomarker discovery.
| Species | Application | Title |
|---|---|---|
MBio A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression. | ||
Oncol Lett Identification of a novel FOXO3‑associated prognostic model in hepatocellular carcinoma |
