Tested Applications
| Positive WB detected in | BGC-823 cells, NCI-H1299 cells |
| Positive IP detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18902-1-AP targets SOX13 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13537 Product name: Recombinant human SOX13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC106038 Sequence: MSMRSPISAQLALDGVGTMVNCTIKSEEKKEPCHEAPQGSATAAEPQPGDPARASQDSADPQAPAQGNFRGSWDCSSPEGNGSPEPKRPGVSEAASGSQEKLDFNRNLKEVVPAIEKLLSSDWKERFLGRNSMEAKDVKGTQESLAEKELQLLVMIHQLSTLRDQLLTAHSEQKNMAAMLFEKQQQQMELARQQQEQIAKQQQQLIQQQHKINLLQQQIQQVNMPYVMIPAFPPSHQPLPVTPDSQLALPIQPIPCKPVEYPLQLLHSPPAPVVKRPGAMATHHPLQEPSQPLNLTAKPKA Predict reactive species |
| Full Name | SRY (sex determining region Y)-box 13 |
| Calculated Molecular Weight | 622 aa, 69 kDa |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | BC106038 |
| Gene Symbol | SOX13 |
| Gene ID (NCBI) | 9580 |
| RRID | AB_10642149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UN79 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SRY-BOX 13(SOX13), identified a sequence homologous more than 60% to the SRY HMG box ,with a centrally located HMG box and an N-terminal leucine zipper motif with a neighboring glutamine stretch called a Q box. SOX13 binds to the consensus HMG-box motif. It's an IDDM-specific human autoantigen, and a gamma-delta-specific gene in the immune system. It can modulates Wnt/TCF activity. This is a rabbit polyclonal raised against part of N-terminal chain of SOX13 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SOX13 antibody 18902-1-AP | Download protocol |
| WB protocol for SOX13 antibody 18902-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SOX13 antibody (Cat# 18902-1-AP) has 6 total citations. These appear in journals including J Exp Clin Cancer Res, Brain, Mol Cell Biochem, Lab Invest, Front Cardiovasc Med, and Bioengineered. Research fields span glioma angiogenesis, neural progenitor development, testicular development, hepatocellular carcinoma metastasis, endothelial inflammation, and breast cancer proliferation/glycolysis. Applications include WB, IHC, IF, and ChIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma. | ||
Brain POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population | ||
Mol Cell Biochem Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development. | ||
Lab Invest Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1.
| ||
Front Cardiovasc Med Sox13 is a novel flow-sensitive transcription factor that prevents inflammation by repressing chemokine expression in endothelial cells
| ||
Bioengineered Sex-determining Region Y-box transcription factor 13 promotes breast cancer cell proliferation and glycolysis by activating the tripartite motif containing 11-mediated Wnt/β-catenin signaling pathway.
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