Tested Applications
| Positive WB detected in | human brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18940-1-AP targets NECAB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13493 Product name: Recombinant human NECAB3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-362 aa of BC047673 Sequence: MACAGLLTVCLLRPPAPQPQPQTPRHPQLAPDPGPAGHTLFQDVFRRADKNDDGKLSFEEFQNYFADGVLSLGELQELFSGIDGHLTDNLETEKLCDYFSEHLGVYRPVLAALESLNRAVLAAMDATKLEYERASKVDQFVTRFLLRETVSQLQALQSSLEGASDTLEAQAHGWRSDAESVEAQSRLCGSRRAGRRALRSVSRSSTWSPGSSDTGRSSEAEMQWRLQVNRLQELIDQLECKAPRLEPLREEDLAKGPDLHILMAQRQVQVAEEGLQDFHRALRCYVDFTGAQSHCLHVSAQKMLDGASFTLYEFWQDEASWRRHQQSPGSKAFQRILIDHLRAPDTLTTVFFPASWWIMNNN Predict reactive species |
| Full Name | N-terminal EF-hand calcium binding protein 3 |
| Calculated Molecular Weight | 362aa,41 kDa; 396aa,44 kDa |
| Observed Molecular Weight | 40-44 kDa |
| GenBank Accession Number | BC047673 |
| Gene Symbol | NECAB3 |
| Gene ID (NCBI) | 63941 |
| RRID | AB_10596622 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96P71 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NECAB3, also named as APBA2BP, NIP1, SYTIP2 and XB51, is a potential substrate of Nek2. NECAB3 inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP). NECAB3 has three isoforms with MW 40 kDa, 44 kDa and 22 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NECAB3 antibody 18940-1-AP | Download protocol |
| WB protocol for NECAB3 antibody 18940-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NECAB3 Polyclonal antibody (Cat# 18940-1-AP) has 1 total citation. It was published in PLoS One (2013), in a study investigating amyloidosis, synucleinopathy, and prion encephalopathy in a neuropathic lysosomal storage disease, exploring the CNS-biomarker potential of peripheral blood. The research falls within the fields of neurodegenerative disease and lysosomal storage disorders. The antibody was applied via IHC on mouse samples.
| Species | Application | Title |
|---|---|---|
PLoS One Amyloidosis, Synucleinopathy, and Prion Encephalopathy in a Neuropathic Lysosomal Storage Disease: The CNS-Biomarker Potential of Peripheral Blood. |
