Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293 cells, human testis tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18989-1-AP targets GLI2-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chamaeleo calyptratus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | GLI family zinc finger 2 |
| Calculated Molecular Weight | 168 kDa |
| Observed Molecular Weight | 167 kDa， 88 kDa |
| GenBank Accession Number | NM_005270 |
| Gene Symbol | GLI2 |
| Gene ID (NCBI) | 2736 |
| RRID | AB_10596479 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10070 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GLI2-Specific antibody 18989-1-AP | Download protocol |
| IHC protocol for GLI2-Specific antibody 18989-1-AP | Download protocol |
| WB protocol for GLI2-Specific antibody 18989-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GLI2-Specific Polyclonal antibody (catalog no. 18989-1-AP) has 64 citations. These appear in journals including Nature Communications, Journal of Advanced Research, Gut Microbes, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cell Death & Disease, International Journal of Biological Sciences, Leukemia, Cell Reports, Journal of Cell Biology, and eLife, among others. Research fields span Hedgehog/GLI2 signaling, cancer stem cells, fibrosis, primary cilia, neurobiology, and developmental biology.
| Species | Application | Title |
|---|---|---|
Nat Commun HSD17B4 deficiency causes dysregulation of primary cilia and is alleviated by acetyl-CoA | ||
J Adv Res Primary cilia protect against intervertebral disc degeneration and spine scoliosis by regulating Hedgehog-P53-mediated cell apoptosis signaling. | ||
Gut Microbes Gut microbiota dysbiosis promotes the development of epithelial ovarian cancer via regulating Hedgehog signaling pathway | ||
Adv Sci (Weinh) Tissue Mechanics and Hedgehog Signaling Crosstalk as a Key Epithelial-Stromal Interplay in Cancer Development | ||
Cell Death Dis Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation |
