BRUNOL6 Polyclonal antibody

BRUNOL6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19078-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CUG-BP- and ETR-3-like factor 6, CELF-6, CELF6, CELF 6, Bruno-like protein 6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 19078-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 19078-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 19078-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 19078-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 19078-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, rat brain tissue, pig brain tissue
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19078-1-AP targets BRUNOL6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4970 Product name: Recombinant human BRUNOL6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 130-481 aa of BC030835 Sequence: RGEDRKLFVGMLGKQQGEEDVRRLFQPFGHIEECTVLRSPDGTSKGCAFVKFGSQGEAQAAIRGLHGSRTMAGASSSLVVKLADTDRERALRRMQQMAGHLGAFHPAPLPLGACGAYTTAILQHQAALLAAAQGPGLGPVAAVAAQMQHVAAFSLVAAPLLPAAAANSPPGSGPGTLPGLPAPIGVNGFGPLTPQTNGQPGSDTLYNNGLSPYPAQSPGVADPLQQAYAGMHHYAAAYPSAYAPVSTAFPQQPSALPQQQREGPEGCNLFIYHLPQEFGDAELIQTFLPFGAVVSAKVFVDRATNQSKCFGFVSFDNPTSAQTAIQAMNGFQIGMKRLKAQLKRPKDANRPY Predict reactive species
Full Name	bruno-like 6, RNA binding protein (Drosophila)
Calculated Molecular Weight	481 aa, 50 kDa
Observed Molecular Weight	50 kDa, 39 kDa
GenBank Accession Number	BC030835
Gene Symbol	BRUNOL6
Gene ID (NCBI)	60677
RRID	AB_2243662
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96J87
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for BRUNOL6 antibody 19078-1-AP	Download protocol
IHC protocol for BRUNOL6 antibody 19078-1-AP	Download protocol
WB protocol for BRUNOL6 antibody 19078-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The BRUNOL6 Polyclonal antibody (Cat# 19078-1-AP) has 1 total citation. It was cited in Cell Death & Disease (2023), in a study investigating how long noncoding RNA LINC01594 inhibits CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis. The research falls within the fields of cancer biology, RNA splicing regulation, and tumor metastasis. The antibody was applied in WB, IHC, and IF using human and mouse samples.

Species	Application	Title
human,mouse	WB,IHC,IF	Cell Death Dis Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis Authors - Bowen Liu View Article