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SULT1A3 Polyclonal antibody

SULT1A3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 19499-1-AP
Publications (2)
Review Product
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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

Sulfotransferase 1A3/1A4, SULT1 A3, SULT1A 3, SULT1A4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of various lysates using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis using 19499-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of Caco-2 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of Caco-2 using 19499-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of COLO 320 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of COLO 320 using 19499-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of MCF-7 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of MCF-7 using 19499-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HepG2 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of HepG2 using 19499-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HT-29 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of HT-29 using 19499-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HuH-7 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    WB analysis of HuH-7 using 19499-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunoprecipitation (IP) experiment of COLO 320 cells using SULT1A3 Polyclonal antibody (19499-1-AP)

    IP experiment of COLO 320 using 19499-1-AP

    IP result of anti-SULT1A3 (IP:19499-1-AP, 4ug; Detection:19499-1-AP 1:200) with COLO 320 cells lysate 2240ug.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human hepatocirrhosis using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human hepatocirrhosis using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human breast cancer using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human breast cancer using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human breast cancer using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1A3 Polyclonal antibody (19499-1-AP)

    IHC staining of human breast cancer using 19499-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inHT-29 cells, Caco-2 cells, COLO 320 cells, HepG2 cells, HuH-7 cells, MCF-7 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19499-1-AP targets SULT1A3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberNM_177552
Gene Symbol SULT1A3
Gene ID (NCBI) 6818
RRIDAB_10642560
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP0DMM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT1A3, also named as STM and SULT1A4, belongs to the sulfotransferase 1 family. SULT1A3 catalyzes the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

Protocols

Product Specific Protocols
IHC protocol for SULT1A3 antibody 19499-1-APDownload protocol
IP protocol for SULT1A3 antibody 19499-1-APDownload protocol
WB protocol for SULT1A3 antibody 19499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SULT1A3 Polyclonal antibody (Cat# 19499-1-AP) has 2 citations. These appear in Nature Metabolism (2022, IF 27.5) and Cancer Letters (2010, IF 11.8). The cited research fields include metabolic reprogramming in tumour progression and sulfotransferase expression in breast cancer, with applications in Western blot and immunohistochemistry.

Automatically generated from the 2 publication citations by Able AI.
SpeciesApplicationTitle
WB

Nat Metab

Altered propionate metabolism contributes to tumour progression and aggressiveness.

Authors - Ana P Gomes
View Article
humanIHC

Cancer Lett

Expression of sulfotransferases and sulfatases in human breast cancer: impact on resveratrol metabolism.

Authors - Miksits Michaela M
View Article
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