HYAL3 Polyclonal antibody

HYAL3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19625-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Hyal 3, HYAL3, Hyaluronidase 3, hyaluronoglucosaminidase 3, LUCA 3, LUCA14, LUCA3, Lung carcinoma protein 3, Minna14

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of human brain using 19625-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19625-1-AP (HYAL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 19625-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 19625-1-AP (HYAL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 19625-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 19625-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 19625-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 19625-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate cancer using 19625-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human prostate cancer using 19625-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human brain tissue, HepG2 cells
Positive IHC detected in	human testis tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19625-1-AP targets HYAL3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8611 Product name: Recombinant human HYAL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC005896 Sequence: MTTQLGPALVLGVALCLGCGQPLPQVPERPFSVLWNVPSAHCEARFGVHLPLNSLGIIANRGQHFHGQNMTIFYKNQLGLYPYFGPRGTAHNGGIPQALPLDRHLALAAYQIHHSLRPGFAGPAVLDWEEWCPLWAGNWGRRRAYQAASWAWAQQVFPDLDPQEQLYKAYTGFEQAARALMEDTLRVAQALRPHGLWGFYHYPACGNGWHSMASNYTGRCHAATLARNTQLHWLWAASSALFPSIYLPPRLPPAHHQAFVRHRLEEAFRVALVGHRHPLPVLAYVRLTHRRSGRFLSQDDLVQSIGVSAALGAAGVVLWGDLSLSSSEEECWHLHDYLVDTLGPYVINVT Predict reactive species
Full Name	hyaluronoglucosaminidase 3
Calculated Molecular Weight	417aa,47 kDa; 463aa,52 kDa
Observed Molecular Weight	47 kDa, 55 kDa
GenBank Accession Number	BC005896
Gene Symbol	HYAL3
Gene ID (NCBI)	8372
RRID	AB_10666437
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43820
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HYAL3 antibody 19625-1-AP	Download protocol
WB protocol for HYAL3 antibody 19625-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The HYAL3 Polyclonal antibody (Cat# 19625-1-AP) has 1 total citation. It appears in Journal of Gastroenterology and Hepatology (IF 3.5), in a 2019 study investigating the suppressive role of HYAL1 and HYAL2 in colorectal cancer metastasis. The cited application was IHC on human samples, placing the research field in oncology, specifically colorectal cancer biology.

Species	Application	Title
human	IHC	J Gastroenterol Hepatol The suppressive role of HYAL1 and HYAL2 in the metastasis of colorectal cancer. Authors - Zhiming Jin View Article