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What's the best dilution for Jagged 2 in ?
Tested Applications
| Positive WB detected in | HT-29 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19696-1-AP targets Jagged 2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | jagged 2 |
| Calculated Molecular Weight | 133 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | NM_002226 |
| Gene Symbol | Jagged 2 |
| Gene ID (NCBI) | 3714 |
| RRID | AB_2878598 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y219 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Jagged 2 antibody 19696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Jagged 2 polyclonal antibody (Cat# 19696-1-AP) has 4 citations. These appear in Bioactive Materials, Carbohydrate Polymers, International Journal of Biological Sciences, and Gene. The associated research fields include osteochondral regeneration via skeletal stem cell exosomes, food allergy mechanisms, esophageal cancer progression through the NOTCH pathway, and Hirschsprung's-associated enterocolitis involving enteric neural precursor cell differentiation via the Notch-1/Jagged-2 pathway.
| Species | Application | Title |
|---|---|---|
Bioact Mater 3D-printed advanced scaffold armed with exosomes derived from human skeletal stem cell identified by single-cell RNA sequencing enhances osteochondral regeneration | ||
Carbohydr Polym Comparative study on the mechanisms of citrus pectin and polygalacturonic acid in alleviating food allergies. | ||
Int J Biol Sci UBE3A activates the NOTCH pathway and promotes esophageal cancer progression by degradation of ZNF185. | ||
Gene ZEB2 alleviates Hirschsprung's-associated enterocolitis by promoting the proliferation and differentiation of enteric neural precursor cells via the Notch-1/Jagged-2 pathway |
