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persephin-Specific Polyclonal antibody

persephin-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 19713-1-AP
Publications (2)
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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

persephin, PSPN, PSP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of various lysates using persephin-Specific Polyclonal antibody (19713-1-AP)

    WB analysis using 19713-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HepG2 cells using persephin-Specific Polyclonal antibody (19713-1-AP)

    WB analysis of HepG2 using 19713-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human brain tissue using persephin-Specific Polyclonal antibody (19713-1-AP)

    IHC staining of human brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using persephin-Specific Polyclonal antibody (19713-1-AP)

    IHC staining of human ovary cancer using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human colon cancer tissue using persephin-Specific Polyclonal antibody (19713-1-AP)

    IHC staining of human colon cancer using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human brain tissue using persephin-Specific Polyclonal antibody (19713-1-AP)

    IHC staining of human brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse brain tissue using persephin-Specific Polyclonal antibody (19713-1-AP)

    IHC staining of mouse brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse brain tissue using persephin-Specific Polyclonal antibody (19713-1-AP)

    IHC staining of mouse brain using 19713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using persephin-Specific Polyclonal antibody (19713-1-AP)

    IF Staining of SH-SY5Y using 19713-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using persephin-Specific antibody (19713-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Flow cytometry (FC) experiment of SH-SY5Y cells using persephin-Specific Polyclonal antibody (19713-1-AP)

    FC experiment of SH-SY5Y using 19713-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Flow cytometry (FC) experiment of HepG2 cells using persephin-Specific Polyclonal antibody (19713-1-AP)

    FC experiment of HepG2 using 19713-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inSH-SY5Y cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human brain tissue, human ovary cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 ul suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19713-1-AP targets persephin-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name persephin
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberNM_004158
Gene Symbol Persephin/PSPN
Gene ID (NCBI) 5623
RRIDAB_10640431
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60542
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSPN, also named as PSP, belongs to the TGF-beta family and GDNF subfamily. PSPN exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. The antibody is specific to PSPN.

Protocols

Product Specific Protocols
IF protocol for persephin-Specific antibody 19713-1-APDownload protocol
IHC protocol for persephin-Specific antibody 19713-1-APDownload protocol
WB protocol for persephin-Specific antibody 19713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The persephin-Specific Polyclonal antibody (catalog number 19713-1-AP) has 2 total citations. These appear in two journals: Signal Transduction and Targeted Therapy and Biological Cell. The associated research fields span oncology and cell biology. One study (2026) examined Nerve Growth Factor signaling's role in limiting lenvatinib response in hepatocellular carcinoma. The other (2017) investigated Vps15p's regulation of cup-shaped organelles containing the eisosome protein Pil1p and extracellular vesicle endocytosis, using the antibody for Western blot (WB).

Automatically generated from the 2 publication citations by Able AI.
SpeciesApplicationTitle

Signal Transduct Target Ther

Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma

Authors - Minghao Xu
View Article
WB

Biol Cell

Vps15p regulates the distribution of cup-shaped organelles containing the major eisosome protein Pil1p to the extracellular fraction required for endocytosis of extracellular vesicles carrying metabolic enzymes.

Authors - Kathryn Stein
View Article
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