Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19741-1-AP targets SYT7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | synaptotagmin VII |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 42-45 kDa |
| GenBank Accession Number | NM_004200 |
| Gene Symbol | SYT7 |
| Gene ID (NCBI) | 9066 |
| RRID | AB_3085583 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43581 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SYT7, also named as PCANAP7 and IPCA-7, belongs to the synaptotagmin family. SYT7 may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SYT7 antibody 19741-1-AP | Download protocol |
| IHC protocol for SYT7 antibody 19741-1-AP | Download protocol |
| WB protocol for SYT7 antibody 19741-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SYT7 Polyclonal antibody (Cat# 19741-1-AP) has 1 citation. It was published in Brain Research Bulletin (2024), where the antibody was used for immunofluorescence in mouse tissue. The study investigated how the double-strand-break repair protein RAD21 homolog/Synaptotagmin-7 alleviates Alzheimer's disease in mice by promoting M2 polarization of microglia, placing the citation in the fields of neurodegeneration, neuroinflammation, and DNA repair.
| Species | Application | Title |
|---|---|---|
Brain Res Bull Double-strand-break repair protein rad21 homolog/Synaptotagmin-7 alleviates Alzheimer's disease in mice by promoting M2 polarization of microglia |
Reviews
What customers say
The single review for this product is negative. The reviewer reported that the antibody failed to detect the expected 42–46 kDa band in any tissue or cell lysate tested. Instead, only an approximately 55 kDa band appeared in mouse brain tissue and cells, along with a ~100 kDa band across all lanes. The antibody was used for Western Blot at a 1:1000 dilution on HEK293T, Neuro2A, mouse primary cerebellar neurons, and various mouse tissues. Overall, the product did not meet the reviewer's expectations regarding the declared molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Olga (Verified Customer) (01-17-2025) | I was disapointed as it did not recognize the band of declared 42-46 kDa in any tissue and cell lysate tested - only the bands of about 55 kDa and mouse brain tissue and cells - and a band of about 100 kDa in all lanes
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