Tested Applications
| Positive WB detected in | mouse small intestine tissue, human kidney tissue, human placenta tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19747-1-AP targets TINAG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | tubulointerstitial nephritis antigen |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | NM_014464 |
| Gene Symbol | TINAG |
| Gene ID (NCBI) | 27283 |
| RRID | AB_10642433 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJW2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TINAG belongs to the peptidase C1 family. TINAG mediates adhesion of proximal tubule epithelial cells via integrins alpha3-beta1 and alphaV-beta3. It is a non catalytic peptidase C1 family protein. Antibodies against TINAG are found in sera of patients with tubulointerstitial nephritis, a rare autoimmune disorder that causes acute and chronic renal injury.It has 2 isoforms produced by alternative splicing. The antibody is specific to TINAG.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TINAG-Specific antibody 19747-1-AP | Download protocol |
| WB protocol for TINAG-Specific antibody 19747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TINAG-Specific Polyclonal antibody (Cat# 19747-1-AP) has 1 total citation. It appears in Translational Cancer Research (2024), in a study modeling new markers for diagnosis and prognosis of pancreatic cancer based on the transition from inflammation to cancer. The cited application was IHC on human samples. The research field is pancreatic oncology and inflammatory-to-malignant transition.
| Species | Application | Title |
|---|---|---|
Transl Cancer Res Modeling of new markers for the diagnosis and prognosis of pancreatic cancer based on the transition from inflammation to cancer |
