Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IHC detected in | mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19789-1-AP targets BAI3-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | brain-specific angiogenesis inhibitor 3 |
| Calculated Molecular Weight | 172 kDa |
| Observed Molecular Weight | 135-140 kDa |
| GenBank Accession Number | NM_001704 |
| Gene Symbol | BAI3 |
| Gene ID (NCBI) | 577 |
| RRID | AB_10666850 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60242 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAI3, also named as KIAA0550, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. BAI3 might be involved in angiogenesis inhibition and suppression of glioblastoma. This antibody is specific to BAI3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BAI3-Specific antibody 19789-1-AP | Download protocol |
| WB protocol for BAI3-Specific antibody 19789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BAI3-Specific Polyclonal antibody (Cat# 19789-1-AP) has 1 citation. It was used in a study published in Behavioral Brain Research (2021), investigating miR-142's role in spatial learning, memory impairment, and apoptosis in APP/PS1 transgenic mice. The antibody was applied via Western blot and immunofluorescence in mouse hippocampal tissue. The research falls within the fields of Alzheimer's disease, neurodegeneration, and hippocampal signaling.
| Species | Application | Title |
|---|---|---|
Behav Brain Res miR-142 downregulation alleviates the impairment of spatial learning and memory, reduces the level of apoptosis, and upregulates the expression of pCaMKII and BAI3 in the hippocampus of APP/PS1 transgenic mice. |
