Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human testis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19893-1-AP targets RFWD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13728 Product name: Recombinant human RFWD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 427-774 aa of BC002574 Sequence: SQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE Predict reactive species |
| Full Name | ring finger and WD repeat domain 3 |
| Calculated Molecular Weight | 774 aa, 85 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC002574 |
| Gene Symbol | RFWD3 |
| Gene ID (NCBI) | 55159 |
| RRID | AB_10639521 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PCD5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RFWD3 antibody 19893-1-AP | Download protocol |
| IHC protocol for RFWD3 antibody 19893-1-AP | Download protocol |
| WB protocol for RFWD3 antibody 19893-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RFWD3 antibody (Cat# 19893-1-AP) has 8 total citations across journals including Nucleic Acids Research, Advanced Science, Cell Reports, Frontiers in Medicine, Frontiers in Oncology, and Histology and Histopathology. Research fields span replication stress and DNA damage response, nucleotide metabolism and chemoresistance in osteosarcoma, E3 ligase function in non-small cell lung cancer, platinum response in ovarian cancer, Rad51 ubiquitination regulation, and bladder cancer progression. Applications include WB, IHC, IF, and ELISA in human samples.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res DOCK7 protects against replication stress by promoting RPA stability on chromatin. | ||
Adv Sci (Weinh) RFWD3 Reprograms Nucleotide Metabolism Through PHGDH to Induce Chemoresistance In Osteosarcoma | ||
Cell Rep Ski2-like helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses. | ||
Front Med Identification of an E3 ligase-encoding gene RFWD3 in non-small cell lung cancer. | ||
Front Oncol The Valproate Mediates Radio-Bidirectional Regulation Through RFWD3-Dependent Ubiquitination on Rad51.
| ||
Front Oncol RFWD3 modulates response to platinum chemotherapy and promotes cancer associated phenotypes in high grade serous ovarian cancer |
