Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, HepG2 cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20048-1-AP targets DAPK2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | death-associated protein kinase 2 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 38-43 kDa |
| GenBank Accession Number | NM_014326 |
| Gene Symbol | DAPK2 |
| Gene ID (NCBI) | 23604 |
| RRID | AB_10667409 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UIK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DAPK2-Specific antibody 20048-1-AP | Download protocol |
| IHC protocol for DAPK2-Specific antibody 20048-1-AP | Download protocol |
| WB protocol for DAPK2-Specific antibody 20048-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DAPK2-Specific Polyclonal antibody (Cat# 20048-1-AP) has 4 citations published in J Pineal Res, Biochem Pharmacol, Biofactors, and J Cancer. The research spans bone biology (melatonin and mechanical unloading), renal pathology (diabetic nephropathy and mitophagy), osteoblast differentiation (autophagy regulation), and oncology (lung adenocarcinoma prognosis). Applications cited include WB and IHC in both human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Pineal Res Melatonin Counteracts Mechanical Unloading-Induced Bone Loss Through YTHDF3-Mediated m(6)A Modification of Dapk2 mRNA.
| ||
Biochem Pharmacol Upregulation of BNIP3 alleviates renal tubular cell injury by activating mitophagy in diabetic nephropathy. | ||
Biofactors VGLL3 Regulates DAPK2-Mediated Autophagy During Osteoblast Differentiation.
| ||
J Cancer Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma |
