Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, HeLa cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse kidney tissue, human spleen tissue, mouse small intestine tissue, human pancreas cancer tissue, human urothelial carcinoma tissue, rat kidney tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20078-1-AP targets MYSM1-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Myb-like, SWIRM and MPN domains 1 |
| Calculated Molecular Weight | 95 kDa |
| Observed Molecular Weight | 81 kDa |
| GenBank Accession Number | NM_001085487 |
| Gene Symbol | MYSM1 |
| Gene ID (NCBI) | 114803 |
| RRID | AB_10664926 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VVJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYSM1-Specific antibody 20078-1-AP | Download protocol |
| IHC protocol for MYSM1-Specific antibody 20078-1-AP | Download protocol |
| IP protocol for MYSM1-Specific antibody 20078-1-AP | Download protocol |
| WB protocol for MYSM1-Specific antibody 20078-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYSM1-Specific Polyclonal antibody (Cat# 20078-1-AP) has 1 citation. It appears in Cell Death Discovery (IF 10.4), published in February 2022. The study investigated MYSM1's role in inducing apoptosis and sensitizing triple-negative breast cancer (TNBC) cells to cisplatin via the RSK3-phospho-BAD pathway. The associated research field is cancer biology, specifically apoptosis and drug sensitivity in breast cancer.
| Species | Application | Title |
|---|---|---|
Cell Death Discov MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway. |
