Tested Applications
| Positive WB detected in | MDA-MB-231 cells, SH-SY5Y cells, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20105-1-AP targets RNF5 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13737 Product name: Recombinant human RNF5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC004155 Sequence: MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFG Predict reactive species |
| Full Name | ring finger protein 5 |
| Calculated Molecular Weight | 180 aa, 20 kDa |
| Observed Molecular Weight | 15-50 kDa |
| GenBank Accession Number | BC004155 |
| Gene Symbol | RNF5 |
| Gene ID (NCBI) | 6048 |
| RRID | AB_3669323 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99942 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The RING (really interesting new gene) finger protein 5 (RNF5) is an ER-associated E3 ubiquitin ligase involved in ER-associated degradation (ERAD) for misfolded proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF5 antibody 20105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RNF5 Polyclonal antibody (Cat# 20105-1-AP) has 3 citations published in Pathology Research & Practice, Biochemical and Biophysical Research Communications, and Apoptosis. The associated research fields include diabetic kidney disease (RNF5-mediated STING degradation), colorectal cancer progression (RNF5-dependent S100A8 degradation), and bladder cancer (STING degradation and macrophage modulation). All citations used the antibody for Western blot in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Pathol Res Pract Ring-finger protein 5 protects against diabetic kidney disease by targeting and degrading STING. | ||
Biochem Biophys Res Commun S100A8 lactylation at K23/K36 reduces RNF5-dependent degradation and promotes colorectal cancer progression. | ||
Apoptosis Targeting LAMP2 synergizes with gemcitabine to inhibit bladder cancer progression via modulating STING degradation and regulating macrophage phenotypes. |
