Tested Applications
| Positive WB detected in | HL-60 cells, MCF-7 cells, PC-3 cells, MDA-MB-231 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20297-1-AP targets P2RY2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14107 Product name: Recombinant human P2RY2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 268-377 aa of BC012104 Sequence: YYSFRSLDLSCHTLNAINMAYKVTRPLASANSCLDPVLYFLAGQSLVRFARDAKPPTGPSPATPARRRLGLRRSDRTDMQRIEDVLGSSEDSRRTESTPAGSENTKDIRL Predict reactive species |
| Full Name | purinergic receptor P2Y, G-protein coupled, 2 |
| Calculated Molecular Weight | 377 aa, 42 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC012104 |
| Gene Symbol | P2RY2 |
| Gene ID (NCBI) | 5029 |
| RRID | AB_3669332 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41231 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P2RY2, also named P2RU1, P2U1, and P2Y2, belongs to the G-protein coupled receptor 1 family. It is a receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Extracellular ATP then acts on purinergic receptors, including P2RY2, to activate proline-rich tyrosine kinase 2 (Pyk2) kinase and transient plasma membrane depolarization.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for P2RY2 antibody 20297-1-AP | Download protocol |
| WB protocol for P2RY2 antibody 20297-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-P2RY2 polyclonal antibody (Cat# 20297-1-AP) has 2 total citations. These appear in International Journal of Biological Macromolecules (IF 8.7) and Tissue and Cell (IF 3.1). Both studies applied the antibody for Western blot in human samples, investigating P2RY2's role in esophageal squamous cell carcinoma progression and as a predictive biomarker in gastric cancer, respectively. The research fields span oncology and molecular biology.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Upregulation of has_circ_0008389 promotes esophageal squamous cell carcinoma progression via miR-761 sponging and P2RY2 mRNA stabilization. | ||
